Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)

Overview

  • Product nameAnti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3]
    See all alpha 1 Sodium Potassium ATPase primary antibodies
  • Description
    Mouse monoclonal [M8-P1-A3] to alpha 1 Sodium Potassium ATPase
  • Tested applicationsSuitable for: IHC-Fr, WB, IHC-P, Inhibition Assay, ICC, ELISA, IP, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Chicken, Dog, Human, Pig, Drosophila melanogaster, Non Human Primates
  • Immunogen

    Other Immunogen Type corresponding to Sheep alpha 1 Sodium Potassium ATPase. Lamb kidney alpha 1 sodium/potassium ATPase.

  • EpitopeThis antibody recognizes an epitope between amino acid residues 496-506 of lamb kidney sodium/potassium ATPase.
  • Positive control
    • WB: canine kidney extract

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberM8-P1-A3
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
WB 1/250. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).
IHC-P 1/100 - 1/2000.
Inhibition Assay Use at an assay dependent concentration.
ICC 1/50 - 1/200.
ELISA 1/40.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/20 - 1/100.

Target

  • FunctionThis is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • Post-translational
    modifications
    Phosphorylation on Tyr-10 modulates pumping activity.
  • Cellular localizationCell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • A1A1 antibody
    • AT1A1 antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • Atpa-1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting subunit alpha 1 antibody
    • BC010319 antibody
    • EC 3.6.3.9 antibody
    • MGC3285 antibody
    • MGC38419 antibody
    • MGC51750 antibody
    • Na K ATPase alpha A catalytic polypeptide antibody
    • Na K ATPase catalytic subunit alpha A protein antibody
    • Na(+)/K(+) ATPase 1 antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase alpha 1 subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na,K ATPase alpha 1 subunit antibody
    • Nkaa1b antibody
    • Sodium potassium ATPase alpha 1 polypeptide antibody
    • Sodium pump 1 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    see all

Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] images

  • Immunocytochemistry/Immunofluorescence analysis of alpha 1 Sodium Potassium ATPase (green) in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature and blocked with 0.3% BSA for 15 minutes at room temperature. Cells were incubated with ab2872 (1:100) for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (1:500) for 30 minutes at room temperature. F-actin (red) was stained with DyLight 594 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.

  • ab2872 staining pig hepatocyte tissue sections by IHC-P.  The section was fixed with Bouins and subjected to heat mediated antigen retrieval (at pH 9) prior to incubating with the primary antibody, diluted 1/2000, for 1 hour at 20°C.  A Cy3® conjugated goat anti-mouse IgG antibody was used as the secondary.

    See Abreview

  • All lanes : Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872) at 1/500 dilution

    Lane 1 : Human brain normal tissue lysate - membrane extract (ab29456)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 110 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
    The 100 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to alpha 1 Sodium Potassium ATPase.
  • Overlay histogram showing HEK293 cells stained with ab2872 (red line). The cells were fixed with 100% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2872, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-1 ab2872 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human kidney tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-1 ab2872 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-1 ab2872 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

References for Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)

This product has been referenced in:
  • Stordal B  et al. Resistance to paclitaxel in a cisplatin-resistant ovarian cancer cell line is mediated by P-glycoprotein. PLoS One 7:e40717 (2012). WB ; Human . Read more (PubMed: 22792399) »
  • Suñé G  et al. Cyclophilin B interacts with sodium-potassium ATPase and is required for pump activity in proximal tubule cells of the kidney. PLoS One 5:e13930 (2010). WB ; Human . Read more (PubMed: 21085665) »

See all 16 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Hepatocytes)
Specification Hepatocytes
Fixative Bouins
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 9,0
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Nov 04 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (Hepatocytes)
Specification Hepatocytes
Fixative Bouins
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 9,0
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Apr 03 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Glioma)
Specification Glioma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Ms. Amy Weaver

Verified customer

Submitted Dec 12 2005

Thank you for your reply. We are going to send you a new batch as soon as we receive it. This is to let you know that I have placed a new order for you - one vial of ab2872 free of charge. For your information your new order number is: 83747. Th...

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Thank you for getting back to me and providing more information about your Western blot protocol. We can offer you a new vial as a free of charge replacement; however it is from the exatly the same batch. Unfortunately, we do not have different bat...

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Thank you for contacting us regarding ab2872 and for taking your time to fill in the on-line Questionnaire. We are very sorry to hear that you are experiencing problem with this antibody. As we understand from your e-mail, you have used HRP mouse m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"