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Other Immunogen Type corresponding to Sheep alpha 1 Sodium Potassium ATPase. Lamb kidney alpha 1 sodium/potassium ATPase.
Our Abpromise guarantee covers the use of ab2872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).|
|IHC-P||1/100 - 1/2000.|
|Inhibition Assay||Use at an assay dependent concentration.|
|ICC||1/50 - 1/200.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||1/20 - 1/100.|
Immunocytochemistry/Immunofluorescence analysis of alpha 1 Sodium Potassium ATPase (green) in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature and blocked with 0.3% BSA for 15 minutes at room temperature. Cells were incubated with ab2872 (1:100) for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (1:500) for 30 minutes at room temperature. F-actin (red) was stained with DyLight 594 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
ab2872 staining pig hepatocyte tissue sections by IHC-P. The section was fixed with Bouins and subjected to heat mediated antigen retrieval (at pH 9) prior to incubating with the primary antibody, diluted 1/2000, for 1 hour at 20°C. A Cy3® conjugated goat anti-mouse IgG antibody was used as the secondary.
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