Anti-alpha 1 Sodium Potassium ATPase (phospho S16) antibody (ab194532)

Overview

  • Product name
    Anti-alpha 1 Sodium Potassium ATPase (phospho S16) antibody
    See all alpha 1 Sodium Potassium ATPase primary antibodies
  • Description
    Rabbit polyclonal to alpha 1 Sodium Potassium ATPase (phospho S16)
  • Host species
    Rabbit
  • Specificity
    ab194532 detects endogenous level of alpha 1 Sodium Potassium ATPase only when phosphorylated at serine 16.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human alpha 1 Sodium Potassium ATPase (phospho S16) conjugated to Keyhole Limpet Haemocyanin (KLH). Peptide sequence around phosphorylation site of serine 16 (A-V-S(p)-E-Q) derived from Human alpha 1 Sodium Potassium ATPase.
    Database link: P05023

  • Positive control
    • 293 cell extract, treated with PMA.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, 49% PBS

    PBS (without Mg2+and Ca2+)
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab194532 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab194532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 113 kDa.

Target

  • Function
    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • Sequence similarities
    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • Post-translational
    modifications
    Phosphorylation on Tyr-10 modulates pumping activity.
  • Cellular localization
    Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • A1A1 antibody
    • AT1A1 antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • Atpa-1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting subunit alpha 1 antibody
    • BC010319 antibody
    • EC 3.6.3.9 antibody
    • MGC3285 antibody
    • MGC38419 antibody
    • MGC51750 antibody
    • Na K ATPase alpha A catalytic polypeptide antibody
    • Na K ATPase catalytic subunit alpha A protein antibody
    • Na(+)/K(+) ATPase 1 antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase alpha 1 subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na,K ATPase alpha 1 subunit antibody
    • Nkaa1b antibody
    • Sodium potassium ATPase alpha 1 polypeptide antibody
    • Sodium pump 1 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    see all

Images

  • All lanes : Anti-alpha 1 Sodium Potassium ATPase (phospho S16) antibody (ab194532) at 1/500 dilution

    Lane 1 : 293 cell extract, treated with PMA
    Lane 2 : 293 cell extract, treated with PMA with antigen-specific peptide

    Predicted band size: 113 kDa

References

ab194532 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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