Anti-alpha 1 Sodium Potassium ATPase (phospho S23) antibody (ab74069)

Overview

  • Product nameAnti-alpha 1 Sodium Potassium ATPase (phospho S23) antibody
    See all alpha 1 Sodium Potassium ATPase primary antibodies
  • Description
    Rabbit polyclonal to alpha 1 Sodium Potassium ATPase (phospho S23)
  • Specificityab74069 detects endogenous levels of alpha 1 Sodium Potassium ATPase only when phosphorylated at serine 23.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human alpha 1 Sodium Potassium ATPase around the phosphorylation site of serine 23 (K-K-SP-K-K).

  • Positive control
    • NIH/3T3 cells and rat brain extracts.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab74069 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab74069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).Can be blocked with alpha 1 Sodium Potassium ATPase peptide (ab180993).
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionThis is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • Post-translational
    modifications
    Phosphorylation on Tyr-10 modulates pumping activity.
  • Cellular localizationCell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • A1A1 antibody
    • AT1A1 antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • Atpa-1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting subunit alpha 1 antibody
    • BC010319 antibody
    • EC 3.6.3.9 antibody
    • MGC3285 antibody
    • MGC38419 antibody
    • MGC51750 antibody
    • Na K ATPase alpha A catalytic polypeptide antibody
    • Na K ATPase catalytic subunit alpha A protein antibody
    • Na(+)/K(+) ATPase 1 antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase alpha 1 subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na,K ATPase alpha 1 subunit antibody
    • Nkaa1b antibody
    • Sodium potassium ATPase alpha 1 polypeptide antibody
    • Sodium pump 1 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    see all

Anti-alpha 1 Sodium Potassium ATPase (phospho S23) antibody images

  • ab74069 at 1/500 dilution staining alpha 1 Sodium Potassium ATPase in NIH/3T3 cells by Immunofluorescence, in the absence or presence of the immunising phosphopeptide.
  • All lanes : Anti-alpha 1 Sodium Potassium ATPase (phospho S23) antibody (ab74069) at 1/500 dilution

    Lane 1 : Rat brain extracts
    Lane 2 : Rat brain extracts with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 113 kDa
    Observed band size : 113 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

References for Anti-alpha 1 Sodium Potassium ATPase (phospho S23) antibody (ab74069)

ab74069 has not yet been referenced specifically in any publications.

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