Anti-alpha 1 Spectrin antibody [17C7] (ab11751)
Key features and details
- Mouse monoclonal [17C7] to alpha 1 Spectrin
- Suitable for: ELISA, IHC-Fr, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-alpha 1 Spectrin antibody [17C7]
See all alpha 1 Spectrin primary antibodies -
Description
Mouse monoclonal [17C7] to alpha 1 Spectrin -
Host species
Mouse -
Specificity
ab11751 is developed against the alpha I spectrin SH3 domain. It does not crossreact with alpha II (nonerythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In NIH 3T3 cells, ab11751 reacts with a 200 kDa protein which is a candidate marker of endocytic vesicles called macropinosomes. -
Tested applications
Suitable for: ELISA, IHC-Fr, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant alpha I spectrin (erythroid spectrin) SH3 domain.
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Positive control
- Erythroid spectrin in red blood cells and cerebellum.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
17C7 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF | (2) |
Use at an assay dependent concentration.
See Abreview. |
WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 280 kDa.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. See Abreview. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 280 kDa. |
Target
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Function
Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. -
Involvement in disease
Defects in SPTA1 are the cause of elliptocytosis type 2 (EL2) [MIM:130600]. EL2 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape.
Defects in SPTA1 are a cause of hereditary pyropoikilocytosis (HPP) [MIM:266140]. HPP is an autosomal recessive disorder characterized by hemolytic anemia, microspherocytosis, poikilocytosis, and an unusual thermal sensitivity of red cells.
Defects in SPTA1 are the cause of spherocytosis type 3 (SPH3) [MIM:270970]; also known as hereditary spherocytosis type 3 (HS3). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH3 is characterized by severe hemolytic anemia. Inheritance is autosomal recessive. -
Sequence similarities
Belongs to the spectrin family.
Contains 3 EF-hand domains.
Contains 1 SH3 domain.
Contains 21 spectrin repeats. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. - Information by UniProt
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Database links
- Entrez Gene: 6708 Human
- Entrez Gene: 20739 Mouse
- Entrez Gene: 289257 Rat
- Omim: 182860 Human
- SwissProt: P02549 Human
- SwissProt: P08032 Mouse
- Unigene: 119825 Human
- Unigene: 200611 Mouse
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Alternative names
- Alpha I spectrin antibody
- EL 2 antibody
- EL2 antibody
see all
Images
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All lanes : Anti-alpha 1 Spectrin antibody [17C7] (ab11751) at 1 µg/ml
Lane 1 : MW marker
Lane 2 : Human Lung lysate
Lane 3 : Mouse Lung lysate
Lane 4 : Rat Brain lysate
Lane 5 : Human Kidney lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP labeled goat anti-mouse IgG
Predicted band size: 280 kDa -
Anti-alpha 1 Spectrin antibody [17C7] (ab11751) at 1/1000 dilution + Human Platelet whole cell lysate at 25 µg
Secondary
HRP-conjugated Goat Anti-mouse IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 280 kDa
Additional bands at: 200 kDa (possible cleavage fragment)
Exposure time: 1 hour
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab11751 has been referenced in 13 publications.
- Dekel E et al. 20S proteasomes secreted by the malaria parasite promote its growth. Nat Commun 12:1172 (2021). PubMed: 33608523
- Lin YS et al. STIM1 Controls the Focal Adhesion Dynamics and Cell Migration by Regulating SOCE in Osteosarcoma. Int J Mol Sci 23:N/A (2021). PubMed: 35008585
- Smith AS et al. MYH9-related disease mutations cause abnormal red blood cell morphology through increased myosin-actin binding at the membrane. Am J Hematol 94:667-677 (2019). PubMed: 30916803
- Wang Y et al. Critical roles of aII spectrin in brain development and epileptic encephalopathy. J Clin Invest 128:760-773 (2018). PubMed: 29337302
- Huang HM et al. Ankyrin-1 Gene Exhibits Allelic Heterogeneity in Conferring Protection Against Malaria. G3 (Bethesda) 7:3133-3144 (2017). WB ; Mouse . PubMed: 28751503