Recombinant Anti-alpha 1,2 Mannosidase IA/MAN1A1 antibody [EPR9957(B)] (ab140613)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9957(B)] to alpha 1,2 Mannosidase IA/MAN1A1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-alpha 1,2 Mannosidase IA/MAN1A1 antibody [EPR9957(B)]
See all alpha 1,2 Mannosidase IA/MAN1A1 primary antibodies -
Description
Rabbit monoclonal [EPR9957(B)] to alpha 1,2 Mannosidase IA/MAN1A1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human alpha 1,2 Mannosidase IA/MAN1A1 aa 600-700 (C terminal). The exact sequence is proprietary.
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Positive control
- HepG2 and 293T cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9957(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa). |
Target
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Function
Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2). -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyl hydrolase 47 family. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 4121 Human
- Omim: 604344 Human
- SwissProt: P33908 Human
- Unigene: 102788 Human
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Alternative names
- 2-alpha-mannosidase IA antibody
- 2-mannosidase IA antibody
- Alpha 1,2 mannosidase IA antibody
see all
Images
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All lanes : Anti-alpha 1,2 Mannosidase IA/MAN1A1 antibody [EPR9957(B)] (ab140613) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab140613 has been referenced in 7 publications.
- Huang S et al. Mitotic phosphorylation inhibits the Golgi mannosidase MAN1A1. Cell Rep 41:111679 (2022). PubMed: 36417860
- Zeng W et al. Elevated N-Glycosylation Contributes to the Cisplatin Resistance of Non-Small Cell Lung Cancer Cells Revealed by Membrane Proteomic and Glycoproteomic Analysis. Front Pharmacol 12:805499 (2021). PubMed: 35002739
- Cheng PW et al. Markers of malignant prostate cancer cells: Golgi localization of a-mannosidase 1A at GM130-GRASP65 site and appearance of high mannose N-glycans on cell surface. Biochem Biophys Res Commun 527:406-410 (2020). PubMed: 32331836
- Frisbie CP et al. Post-ER Stress Biogenesis of Golgi Is Governed by Giantin. Cells 8:N/A (2019). PubMed: 31847122
- Zhang X et al. GORASP2/GRASP55 collaborates with the PtdIns3K UVRAG complex to facilitate autophagosome-lysosome fusion. Autophagy 15:1787-1800 (2019). PubMed: 30894053
- Legler K et al. Reduced mannosidase MAN1A1 expression leads to aberrant N-glycosylation and impaired survival in breast cancer. Br J Cancer 118:847-856 (2018). PubMed: 29381688
- Casey CA et al. Study of Ethanol-Induced Golgi Disorganization Reveals the Potential Mechanism of Alcohol-Impaired N-Glycosylation. Alcohol Clin Exp Res 40:2573-2590 (2016). PubMed: 27748959