Overview

  • Product name
    Anti-alpha 1,2 Mannosidase IA antibody
    See all alpha 1,2 Mannosidase IA primary antibodies
  • Description
    Rabbit polyclonal to alpha 1,2 Mannosidase IA
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    TLQKLPEEIQRDILLEK

    with N-terminal added cysteine, conjugated to KLH, corresponding to amino acids 138-154 of Human alpha 1,2 Mannosidase IA

  • Positive control
    • Whole extract of Mouse NIH 3T3 cells; Human HepG2 cells; Rat NRK cells

Properties

Applications

Our Abpromise guarantee covers the use of ab51262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 73 kDa.
ICC/IF Use a concentration of 5 - 10 µg/ml.

Target

  • Function
    Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2).
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyl hydrolase 47 family.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2-alpha-mannosidase IA antibody
    • 2-mannosidase IA antibody
    • Alpha 1,2 mannosidase IA antibody
    • Alpha-1 antibody
    • EC 3.2.1.113 antibody
    • HUMM3 antibody
    • HUMM9 antibody
    • MA1A1_HUMAN antibody
    • Man(9) alpha mannosidase antibody
    • Man(9)-alpha-mannosidase antibody
    • MAN1A1 antibody
    • MAN9 antibody
    • Man9 mannosidase antibody
    • Man9-mannosidase antibody
    • Mannosidase alpha class 1A member 1 antibody
    • Mannosyl-oligosaccharide 1 antibody
    • Mannosyl-oligosaccharide 1,2 alpha mannosidase IA antibody
    • OTTHUMP00000017107 antibody
    • Processing alpha 1,2 mannosidase IA antibody
    • Processing alpha-1 antibody
    see all

Anti-alpha 1,2 Mannosidase IA antibody images

  • Anti-alpha 1,2 Mannosidase IA antibody (ab51262) at 1/1000 dilution + Human colon whole cell lysate at 40 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 73 kDa
    Observed band size : 73 kDa


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Praveen Agrawal

    See Abreview

References for Anti-alpha 1,2 Mannosidase IA antibody (ab51262)

This product has been referenced in:
  • Agrawal P  et al. Mapping posttranscriptional regulation of the human glycome uncovers microRNA defining the glycocode. Proc Natl Acad Sci U S A 111:4338-43 (2014). Read more (PubMed: 24591635) »

See 1 Publication for this product

Product Wall

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Colon cell line)
Specification
Colon cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Dr. Praveen Agrawal

Verified customer

Submitted May 13 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up