Anti-alpha 1b Adrenergic Receptor antibody (ab84405)


  • Product nameAnti-alpha 1b Adrenergic Receptor antibody
    See all alpha 1b Adrenergic Receptor primary antibodies
  • Description
    Rabbit polyclonal to alpha 1b Adrenergic Receptor
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A 15 amino acid peptide from the C terminal residues of human alpha 1b Adrenergic Receptor (NM_000679; P35368).

  • Positive control
    • Human fetal brain lysates; human heart tissue.


  • FormLyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab84405 is purified by a peptide affinity column.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab84405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/200 - 1/1000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/500.


  • FunctionThis alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily.
  • Cellular localizationNucleus membrane. Cell membrane. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADA1B_HUMAN antibody
    • ADRA 1 antibody
    • Adra 1b antibody
    • ADRA1 antibody
    • ADRA1B antibody
    • Adrenergic alpha 1B receptor antibody
    • Adrenergic receptor alpha 1b antibody
    • Adrenoceptor alpha 1B antibody
    • Alpha 1 adrenergic receptor antibody
    • Alpha 1B adrenoceptor antibody
    • Alpha 1B adrenoreceptor antibody
    • Alpha-1B adrenergic receptor antibody
    • Alpha-1B adrenoceptor antibody
    • Alpha-1B adrenoreceptor antibody
    • Alpha1 adrenergic receptor antibody
    • Alpha1B adrenergic receptor antibody
    • Alpha1BAR antibody
    see all

Anti-alpha 1b Adrenergic Receptor antibody images

  • Anti-alpha 1b Adrenergic Receptor antibody (ab84405) at 1/500 dilution + human fetal brain lysates

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • ab84405, at a 1/100 dilution, staining alpha 1b Adrenergic Receptor in formalin fixed, paraffin embedded human heart tissue by Immunohistochemistry.
  • ICC/IF image of ab84450 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84450, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-alpha 1b Adrenergic Receptor antibody (ab84405)

This product has been referenced in:
  • Mitrano DA  et al. D1-dopamine and a1-adrenergic receptors co-localize in dendrites of the rat prefrontal cortex. Neuroscience 258:90-100 (2014). ICC/IF ; Human . Read more (PubMed: 24231738) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step (agent) for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako High pH
Sample Human Tissue sections (Tendon)
Specification Tendon
Permeabilization No
Fixative Formaldehyde

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Verified customer

Submitted Aug 18 2014

Thank you for your response. Regrettably, ab12923, ab12935 and ab13298 have been discontinued so currently we do no have stock of them in our catalogue. I have conducted a search for your and found some alternatives products for these targets. ...

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