Recombinant
RabMAb

Anti-alpha 1b Adrenergic Receptor antibody [EPR10336] (ab169523)

Overview

  • Product name
    Anti-alpha 1b Adrenergic Receptor antibody [EPR10336]
    See all alpha 1b Adrenergic Receptor primary antibodies
  • Description
    Rabbit monoclonal [EPR10336] to alpha 1b Adrenergic Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human alpha 1b Adrenergic Receptor aa 150-250. The exact sequence is proprietary.
    Database link: P35368

  • Positive control
    • Fetal brain, PC-3 and HeLa lysates, HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10336
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
    • Sequence similarities
      Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1B sub-subfamily.
    • Cellular localization
      Nucleus membrane. Cell membrane. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADA1B_HUMAN antibody
      • ADRA 1 antibody
      • Adra 1b antibody
      • ADRA1 antibody
      • ADRA1B antibody
      • Adrenergic alpha 1B receptor antibody
      • Adrenergic receptor alpha 1b antibody
      • Adrenoceptor alpha 1B antibody
      • Alpha 1 adrenergic receptor antibody
      • Alpha 1B adrenoceptor antibody
      • Alpha 1B adrenoreceptor antibody
      • Alpha-1B adrenergic receptor antibody
      • Alpha-1B adrenoceptor antibody
      • Alpha-1B adrenoreceptor antibody
      • Alpha1 adrenergic receptor antibody
      • Alpha1B adrenergic receptor antibody
      • Alpha1BAR antibody
      see all

    Images

    • All lanes : Anti-alpha 1b Adrenergic Receptor antibody [EPR10336] (ab169523) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : PC-3 lysate
      Lane 3 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 57 kDa

    • Immunofluorescent analysis of HeLa cells labeling alpha 1b Adrenergic Receptor with ab169523 at 1/250.
    • Flow Cytometrical analysis of HeLa cells with ab169523 antibody at a dilution of 1/100 (red) or a rabbit IgG negative (green).

    References

    This product has been referenced in:
    • Gao X  et al. Asymmetrical ligand-induced cross-regulation of chemokine (C-X-C motif) receptor 4 by a1-adrenergic receptors at the heteromeric receptor complex. Sci Rep 8:2730 (2018). Read more (PubMed: 29426850) »
    • Albee LJ  et al. a1-Adrenergic Receptors Function Within Hetero-Oligomeric Complexes With Atypical Chemokine Receptor 3 and Chemokine (C-X-C motif) Receptor 4 in Vascular Smooth Muscle Cells. J Am Heart Assoc 6:N/A (2017). Read more (PubMed: 28862946) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab169523.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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