alpha 2 Catenin protein (Tagged-His Tag) (ab92065)
This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
Our Abpromise guarantee covers the use of ab92065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Alpha catenin related proteinAlpha N cateninAlpha N-catenin
- Alpha-catenin-related proteinCadherin associated protein relatedCancer/testis antigen 114CAP RCAPRCatenin (cadherin associated protein) alpha 2Catenin alpha 2Catenin alpha-2CT114CTNA2_HUMANCTNN A2CTNNA 2CTNNA2CTNRDKFZp686H02198
alpha 2 Catenin protein (Tagged-His Tag) images
The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.
References for alpha 2 Catenin protein (Tagged-His Tag) (ab92065)
ab92065 has not yet been referenced specifically in any publications.