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alpha 2 Catenin protein (Tagged-His Tag) (ab92065)

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Overview

  • Product namealpha 2 Catenin protein (Tagged-His Tag)See all alpha 2 Catenin proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 681-905 of Human alpha 2 Catenin isoform 2, with N terminal His tag, 225aa, MWt 26kDa, Swissprot: P26232-2
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitution with 80 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesAKIAEQVEIFHQEKSKLDAEVAKWDDSGNDIIVLAKQMCM IMMEMTDFTRG KGPLKNTSDVINAAKKIAEAGSRMDKL ARAVADQCPDSACKQDLLAYLQRI ALYCHQLNICSKVK AEVQNLGGELIVSGLDSATSLIQAAKNLMNAVVLTVK A SYVASTKYQKVYGTAAVNSPVVSWKMKAPEKKPLVKREKP EEFQTRVRRG SQKKHISPVQALSEFKAMDSF
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92065 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Alpha catenin related proteinAlpha N cateninAlpha N-catenin
        Alpha-catenin-related proteinCadherin associated protein relatedCancer/testis antigen 114CAP RCAPRCatenin (cadherin associated protein) alpha 2Catenin alpha 2Catenin alpha-2CT114CTNA2_HUMANCTNN A2CTNNA 2CTNNA2CTNRDKFZp686H02198
      see all
  • FunctionMay function as a linker between cadherin adhesion receptors and the cytoskeleton to regulate cell-cell adhesion and differentiation in the nervous system. Regulates morphological plasticity of synapses and cerebellar and hippocampal lamination during development. Functions in the control of startle modulation.
  • Sequence similaritiesBelongs to the vinculin/alpha-catenin family.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell projection > axon. Recruited to the nucleus upon ZNF639 overexpression.

    • Target information above from: UniProt accession P26232 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    alpha 2 Catenin protein (Tagged-His Tag) images

    • SDS-PAGE - alpha 2 Catenin protein (Tagged-His Tag) (ab92065)
      SDS-PAGE - alpha 2 Catenin protein (Tagged-His Tag) (ab92065)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for alpha 2 Catenin protein (Tagged-His Tag) (ab92065)

    ab92065 has not yet been referenced specifically in any publications.

    Publishing research using ab92065 ? Please let us know so that we can cite the reference in this datasheet

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