You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

alpha 2 Macroglobulin Human ELISA Kit (ab108888)

Submit an Abreview Q&A (2)

Overview

  • Product namealpha 2 Macroglobulin Human ELISA KitSee all alpha 2 Macroglobulin kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    = 1 µg/ml
  • Range
    1.25 µg/ml - 40 µg/ml
  • Recovery

    98 %

  • Assay time
    3h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Alpha 2 Macroglobulin is a major serum protein with diverse functions, including inhibition of protease activity and binding of growth factors, cytokines, and disease factors. Increased serum alpha-2-Macroglobulin has been suggested to be associated with multiple sclerosis (MS), glomerular disease, and with liver diseases.

    ab108888 is designed for detection of human alpha 2 Macroglobulin in plasma, and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures human alpha 2 Macroglobulin in less than 3 hours. A polyclonal antibody specific for human alpha 2 Macroglobulin has been pre-coated onto a 96-well microplate with removable strips. Alpha 2 Macroglobulin in standards and samples is competed by a biotinylated alpha 2 Macroglobulin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsCompetitive ELISA more details

Properties

  • FunctionIs able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • Tissue specificitySecreted in plasma.
  • Sequence similaritiesBelongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • Developmental stageContrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P01023 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5A2mA2MD
        A2MG_HUMANAlpha-2-MAlpha-2-macroglobulinC3 and PZP-like alpha-2-macroglobulin domain-containing protein 5CPAMD5FWP007Pregnancy zone proteinS863-7
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108888 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Competitive ELISA cELISA

      alpha 2 Macroglobulin Human ELISA Kit images

      • Sandwich ELISA - alpha 2 Macroglobulin Human ELISA Kit (ab108888)
        Sandwich ELISA - alpha 2 Macroglobulin Human ELISA Kit (ab108888)

      Protocols

      References for alpha 2 Macroglobulin Human ELISA Kit (ab108888)

      ab108888 has not yet been referenced specifically in any publications.

      Publishing research using ab108888 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab108888 Submit a Question for ab108888

      Displaying 1 - 2 of 2 results for Abreviews and Q&A

      10
      Votes: Highest First

      I will be using samples that are pre-anticoagulated (states that the anticoagulation is performed with ATC). The protocol states that sodium citrate, EDTA or heparin can be used with the kit. Will there be an issue with using these samples with the kit?...

      Read More

      Thank you for contacting us.

      Unfortunately the laboratory has no data regarding on ATC samples, and cannot give a recommendation here.

      Please do not hesitate to contact us if you need any more advice or information.

      What volume should the standard be reconstituted with? Lot # GR100954-1

      Thank you very much for your call yesterday and for your patience while I've been in touch with my colleagues regarding this standard reconstitution volume.

      For this lot, the standard vial contains 160 ug of human alpha-2-macroglobulin. So, to g...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"