Anti-alpha 2 Macroglobulin antibody [2D9] (ab36995)

Overview

  • Product nameAnti-alpha 2 Macroglobulin antibody [2D9]See all alpha 2 Macroglobulin primary antibodies ...
  • Description
    Mouse monoclonal [2D9] to alpha 2 Macroglobulin
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Alpha-2-macroglobulin protein purified from Human plasma.

  • Positive control
    • Human plasma.
  • General notesWe have received mixed feedback from our customers regarding the IHC-P application. Please contact the scientific support team for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab36995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 178 kDa (predicted molecular weight: 163 kDa).
ELISA Use at an assay dependent dilution.

Target

  • FunctionIs able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • Tissue specificitySecreted in plasma.
  • Sequence similaritiesBelongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • Developmental stageContrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • A2M antibody
    • A2MG_HUMAN antibody
    • Alpha 2 M antibody
    • Alpha 2M antibody
    • Alpha-2-M antibody
    • Alpha-2-macroglobulin antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 antibody
    • CPAMD5 antibody
    • DKFZp779B086 antibody
    • FWP007 antibody
    • S863 7 antibody
    see all

Anti-alpha 2 Macroglobulin antibody [2D9] images

  • All lanes : Anti-alpha 2 Macroglobulin antibody [2D9] (ab36995)

    Lane 1 : Alpha 2 Macroglobulin isolated from Human plasma
    Lane 2 : Human plasma

    Lysates/proteins at 50 µg per lane.

    Secondary
    anti-mouse IgG -HRP

    Predicted band size : 163 kDa
    Observed band size : ~178 kDa (why is the actual band size different from the predicted?)
    Additional bands at : >200 kDa (possible non-specific binding).

References for Anti-alpha 2 Macroglobulin antibody [2D9] (ab36995)

This product has been referenced in:
  • Lund J  et al. ADAMDEC1 is a metzincin metalloprotease with dampened proteolytic activity. J Biol Chem 288:21367-75 (2013). Read more (PubMed: 23754285) »
  • Yoo SW  et al. A Large Network of Interconnected Signaling Pathways in Human Ovarian Follicles is Supported by the Gene Expression Activity of the Granulosa Cells. Reprod Sci 18:476-84 (2011). Read more (PubMed: 21558463) »

See all 4 Publications for this product

Product Wall

Thank you for your patience.

The lab let me know the following:

We use a common method of plasma sample preparation.
1. Collect human blood
2. Collect plasma after centrifugation (Plasma is the centrifugation resulting superna...

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Thank you for contacting Abcam yesterday.

Theimmunogen for ab36995 is a 2-macroglobulin protein purified from Human plasma. We are sorry. We’ve not mapped the epitope for this antibody and so I cannot say whether it would bind in the C-...

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Thank you for your reply.

Indeed we do not have another mouseanti alpha 2 Macroglobulin antibody which would be tested in Western blot already. We have the ab116593, which however has only been tested in ELISA, IP and RIA.

There is ...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital ...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (brain)
Specification brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA (pH8)
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Rachel Barker

Verified customer

Submitted Oct 28 2010

Application Western blot
Sample Human Tissue lysate - whole (brain)
Loading amount 30 µg
Specification brain
Gel Running Conditions Reduced Denaturing (4-20% Tris-HCl gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Rachel Barker

Verified customer

Submitted Oct 28 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"