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Anti-alpha 2 Macroglobulin antibody [2D9] (ab36995)

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Overview

Product name

Anti-alpha 2 Macroglobulin antibody [2D9]
See all alpha 2 Macroglobulin products (19) ...

Description

Mouse monoclonal [2D9] to alpha 2 Macroglobulin

Tested applications

WB, ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Human

Immunogen

Alpha-2-macroglobulin protein purified from Human plasma.

Positive control

Human plasma.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.03% Sodium Azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

2D9

Isotype

IgG1

Light chain type

kappa

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Other protease inhibitors
Cardiovascular >> Blood >> Serum Proteins

  • Western blot - alpha 2 Macroglobulin antibody [2D9] (ab36995)Western blot - alpha 2 Macroglobulin antibody [2D9] (ab36995) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab36995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Tissue specificity

Secreted in plasma.

Sequence similarities

Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.

Developmental stage

Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.

Cellular localization

Secreted.

Target information above from: UniProt accessionP01023 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • A2M antibody
  • A2MG_HUMAN antibody
  • Alpha 2 M antibody
  • Alpha 2M antibody
  • Alpha-2-M antibody
  • Alpha-2-macroglobulin antibody
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 antibody
  • CPAMD5 antibody
  • DKFZp779B086 antibody
  • FWP007 antibody
  • S863 7 antibody
see all

Database links

Anti-alpha 2 Macroglobulin antibody [2D9] images:

  Western blot - alpha 2 Macroglobulin antibody [2D9] (ab36995)

Western blot - alpha 2 Macroglobulin antibody [2D9] (ab36995)

All lanes : Anti-alpha 2 Macroglobulin antibody [2D9] (ab36995)

Lane 1 : Alpha 2 Macroglobulin isolated from Human plasma
Lane 2 : Human plasma

Lysates/proteins at 50 µg per lane.

Secondary
anti-mouse IgG -HRP

Predicted band size : 163 kDa
Observed band size : ~178 kDa (why is the actual band size different from the predicted?)
Additional bands at : >200 kDa (possible non-specific binding).

Alpha-2-Macroglobulin is a 720 kDa homotetrameric glycoprotein composed of four identical 180kDa subunits. The predicted band size of 163 kDa is the MW of the unprocessed precursor.

References for Anti-alpha 2 Macroglobulin antibody [2D9] (ab36995)

This product has been referenced in:

  • Yoo SW  et al. A Large Network of Interconnected Signaling Pathways in Human Ovarian Follicles is Supported by the Gene Expression Activity of the Granulosa Cells. Reprod Sci 18:476-84 (2011).Read more (PubMed: 21558463) »
  • Raymond WW  et al. Alpha2-macroglobulin capture allows detection of mast cell chymase in serum and creates a reservoir of angiotensin II-generating activity. J Immunol 182:5770-7 (2009). WB; Human.Read more (PubMed: 19380825) »

See all 3 publications for this product

Publishing research using ab36995? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"