Anti-alpha 2 Macroglobulin antibody (Biotin) (ab7338)

Overview

  • Product nameAnti-alpha 2 Macroglobulin antibody (Biotin)See all alpha 2 Macroglobulin primary antibodies ...
  • Description
    Goat polyclonal to alpha 2 Macroglobulin (Biotin)
  • ConjugationBiotin
  • Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • Tested applicationsImmunomicroscopy, IP, WB, Dot Blot, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Alpha-2-Macroglobulin [Human Plasma]

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab7338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
IP
WB
Dot Blot
ELISA
IHC-Fr
  • Application notesImmunoblotting (western or dot blot)
    ELISA (1:9,000 to 1:36,000)
    Immunohistochemistry
    Immunomicroscopy
    as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
  • Target

    • FunctionIs able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
    • Tissue specificitySecreted in plasma.
    • Sequence similaritiesBelongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
    • Developmental stageContrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • A2M antibody
      • A2MG_HUMAN antibody
      • Alpha 2 M antibody
      • Alpha 2M antibody
      • Alpha-2-M antibody
      • Alpha-2-macroglobulin antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 antibody
      • CPAMD5 antibody
      • DKFZp779B086 antibody
      • FWP007 antibody
      • S863 7 antibody
      see all

    References for Anti-alpha 2 Macroglobulin antibody (Biotin) (ab7338)

    This product has been referenced in:
    • Jensen PE  et al. Significantly increased fractions of transformed to total alpha2-macroglobulin concentrations in plasma from patients with multiple sclerosis. Biochim Biophys Acta 1690:203-7 (2004). ELISA . Read more (PubMed: 15511627) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. As these 4 antibodies are all polyclonal antibodies, each will recognize multiple epitopes. If you have any more questions, please do contact us again.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"