RabMAb

Anti-alpha 2 Macroglobulin antibody [EPR4432] (ab109422)

Overview

  • Product nameAnti-alpha 2 Macroglobulin antibody [EPR4432]
    See all alpha 2 Macroglobulin primary antibodies
  • Description
    Rabbit monoclonal [EPR4432] to alpha 2 Macroglobulin
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in Human alpha 2 Macroglobulin.

  • Positive control
    • Human plasma lysate; Human liver tissue
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab109422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 163 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionIs able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
    • Tissue specificitySecreted in plasma.
    • Sequence similaritiesBelongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
    • Developmental stageContrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • A2m antibody
      • A2MG_HUMAN antibody
      • Alpha 2 M antibody
      • Alpha 2M antibody
      • Alpha-2-M antibody
      • Alpha-2-macroglobulin antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 antibody
      • CPAMD5 antibody
      • DKFZp779B086 antibody
      • FWP007 antibody
      • S863 7 antibody
      see all

    Anti-alpha 2 Macroglobulin antibody [EPR4432] images

    • Anti-alpha 2 Macroglobulin antibody [EPR4432] (ab109422) at 1/1000 dilution + Human plasma lysate at 10 µg

      Predicted band size : 163 kDa
    • ab109422, at 1/500 dilution, staining alpha 2 Macroglobulin in paraffin-embedded Human liver by Immunohistochemistry.

    References for Anti-alpha 2 Macroglobulin antibody [EPR4432] (ab109422)

    ab109422 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"