Anti-alpha 2 Macroglobulin antibody [EPR4432] (ab109422)


  • Product name
    Anti-alpha 2 Macroglobulin antibody [EPR4432]
    See all alpha 2 Macroglobulin primary antibodies
  • Description
    Rabbit monoclonal [EPR4432] to alpha 2 Macroglobulin
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human alpha 2 Macroglobulin.

  • Positive control
    • Human plasma lysate; Human liver tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab109422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 163 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
    • Tissue specificity
      Secreted in plasma.
    • Sequence similarities
      Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
    • Developmental stage
      Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • A2m antibody
      • A2MG_HUMAN antibody
      • Alpha 2 M antibody
      • Alpha 2M antibody
      • Alpha-2-M antibody
      • Alpha-2-macroglobulin antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 antibody
      • CPAMD5 antibody
      • DKFZp779B086 antibody
      • FWP007 antibody
      • S863 7 antibody
      see all

    Anti-alpha 2 Macroglobulin antibody [EPR4432] images

    • Anti-alpha 2 Macroglobulin antibody [EPR4432] (ab109422) at 1/1000 dilution + Human plasma lysate at 10 µg

      Predicted band size : 163 kDa
    • ab109422, at 1/500 dilution, staining alpha 2 Macroglobulin in paraffin-embedded Human liver by Immunohistochemistry.

    References for Anti-alpha 2 Macroglobulin antibody [EPR4432] (ab109422)

    ab109422 has not yet been referenced specifically in any publications.

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