Anti-alpha 2 Macroglobulin antibody (ab58703)

Overview

  • Product nameAnti-alpha 2 Macroglobulin antibodySee all alpha 2 Macroglobulin primary antibodies ...
  • Description
    Rabbit polyclonal to alpha 2 Macroglobulin
  • Tested applicationsWB, ELISA, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to C terminal residues of human alpha 2 Macroglobulin.

Properties

Applications

Our Abpromise guarantee covers the use of ab58703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
ICC
  • Application notesELISA: Use at an assay dependent dilution.
    ICC: 1/500.
    IHC-P: Use at a concentration of 2 µg/ml.
    WB: Use at an assay dependent concentration. Predicted molecular weight: 163 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIs able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
    • Tissue specificitySecreted in plasma.
    • Sequence similaritiesBelongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
    • Developmental stageContrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • A2M antibody
      • A2MG_HUMAN antibody
      • Alpha 2 M antibody
      • Alpha 2M antibody
      • Alpha-2-M antibody
      • Alpha-2-macroglobulin antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 antibody
      • CPAMD5 antibody
      • DKFZp779B086 antibody
      • FWP007 antibody
      • S863 7 antibody
      see all

    Anti-alpha 2 Macroglobulin antibody images

    • Ab58703 staining alpha 2 Macroglobulin in human liver.
      Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
    • ab58703 staining alpha 2 Macroglobulin in rat decidual cells by Immunocytochemistry. Cells were fixed with Methanol, permeabilized with PBS, 5% BSA, 0.1% Triton X-100 and 0.2% Tween-20 and blocking with 5% BSA was performed for 15 minutes at RT. Samples were incubated with primary antibody (1/500) in PBS for 14 hours at 4°C. A commercially available Avidin–biotin alkaline phosphatase complex technique was used.

      See Abreview

    • All lanes : Anti-alpha 2 Macroglobulin antibody (ab58703) at 1/1000 dilution

      Lane 1 : lysates prepared from rat spinal cord tissues (control group)
      Lane 2 : lysates prepared from rat spinal cord tissues (control group)
      Lane 3 : lysates prepared from rat spinal cord tissues (control group)
      Lane 4 : lysates prepared from rat spinal cord tissues (encephalomyelitis group)
      Lane 5 : lysates prepared from rat spinal cord tissues (encephalomyelitis group)
      Lane 6 : lysates prepared from rat spinal cord tissues (encephalomyelitis group)

      Lysates/proteins at 30 µg per lane.

      Secondary
      goat anti-rabbit IgG coupled to horseradish peroxidase

      Predicted band size : 163 kDa
      Observed band size : 163 kDa
      Additional bands at : 60 kDa (possible cleavage fragment),80 kDa (possible cleavage fragment).

      Image from Jain MR et al, Proteome Sci. 2009 Jul 16;7:25, Fig 2.

    • Anti-alpha 2 Macroglobulin antibody (ab58703) at 1 µg/ml + Human alpha 2 Macroglobulin full length protein (ab77935) at 0.1 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 3 minutes

    References for Anti-alpha 2 Macroglobulin antibody (ab58703)

    This product has been referenced in:
    • Tomazic PV  et al. Nasal mucus proteomic changes reflect altered immune responses and epithelial permeability in patients with allergic rhinitis. J Allergy Clin Immunol N/A:N/A (2013). WB ; Human . Read more (PubMed: 24290289) »
    • Jain MR  et al. Altered proteolytic events in experimental autoimmune encephalomyelitis discovered by iTRAQ shotgun proteomics analysis of spinal cord. Proteome Sci 7:25 (2009). WB ; Rat . Read more (PubMed: 19607715) »

    See all 3 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Tissue lysate - whole (left ventricular heart tissue)
    Loading amount 65 µg
    Specification left ventricular heart tissue
    Gel Running Conditions Reduced Denaturing (10-20% gradient)
    Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Dawn Bowles

    Verified customer

    Submitted Jan 10 2013

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    Application Western blot
    Sample Horse Serum (normal)
    Loading amount 7 µg
    Specification normal
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 20 2011

    Application Immunocytochemistry
    Sample Rat Cultured Cells (Decidual Cells)
    Specification Decidual Cells
    Fixative Methanol
    Permeabilization Yes - PBS, 5% BSA, 0.1% Triton X-100 and 0.2% Tween-20
    Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Dr. Bruno Fonseca

    Verified customer

    Submitted Sep 03 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"