Overview

  • Product nameAnti-alpha 2 Macroglobulin antibody
    See all alpha 2 Macroglobulin primary antibodies
  • Description
    Goat polyclonal to alpha 2 Macroglobulin
  • Tested applicationsICC/IF, WB, IP, ELISA, Conjugation, Dot Blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Alpha-2-Macroglobulin [Human Plasma]

  • Positive control
    • ELISA/WB: recombinant protein (human) ICC/IF: Malme-3m cell line

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab7337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use at an assay dependent concentration. Use under non reducing condition. Detects a band of approximately 726 kDa.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

This product has been assayed against 1.0 ug of ?2-Macroglobulin [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) ab6721 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid] as a substrate for 30 minutes at room temperature.

Conjugation Use at an assay dependent concentration.
Dot Blot Use at an assay dependent concentration.

Target

  • FunctionIs able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • Tissue specificitySecreted in plasma.
  • Sequence similaritiesBelongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • Developmental stageContrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • A2M antibody
    • A2MG_HUMAN antibody
    • Alpha 2 M antibody
    • Alpha 2M antibody
    • Alpha-2-M antibody
    • Alpha-2-macroglobulin antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 antibody
    • CPAMD5 antibody
    • DKFZp779B086 antibody
    • FWP007 antibody
    • S863 7 antibody
    see all

Anti-alpha 2 Macroglobulin antibody images

  • ICC/IF image of ab7337 stained Malme-3m cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7337, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96931, DyLight® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-alpha 2 Macroglobulin antibody (ab7337)

This product has been referenced in:
  • Burgess EF  et al. Prostate cancer serum biomarker discovery through proteomic analysis of alpha-2 macroglobulin protein complexes. Proteomics Clin Appl 2:1223 (2008). WB, IP ; Human . Read more (PubMed: 20107526) »

See 1 Publication for this product

Product Wall

Thank you for contacting Abcam.

The concentration of this antibody is 10mg/ml when restored in 5 ml of water as directed.

Please feel free to contact me for additional assistance regarding this issue.

Thank you for contacting us. Please find the Certificate of Compliance for this lot attached. Unfortunately, we do not provide Certificates of Analysis for our antibody products. Please let me know if you need any additional information or assistance.

Thank you for contacting Abcam.

I did a sequence alignment with the human and mouse alpha 2 Macroglobulin sequences and there was only a 59% level of homology between the 2, usually we look for about 85%homology before we would recommend tryi...

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Our records show we used the purified protein as the positive control and the antibody recognizes the protein at 726kDa in non-reducing SDS-PAGE. Unfortunately we do not have an image or other details available. I hope this information will alrea...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"