The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at a concentration of 5 µg/ml (for coating) or 0.2 µg/ml (for detection).
WB: Use at a concentration of 0.2 µg/ml. Predicted molecular weight: 164 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionIs able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Tissue specificitySecreted in plasma.
Sequence similaritiesBelongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
Developmental stageContrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.