Anti-Alpha 2 Antiplasmin antibody [710-1E-94] (ab30994)

Overview

  • Product nameAnti-Alpha 2 Antiplasmin antibody [710-1E-94]
    See all Alpha 2 Antiplasmin primary antibodies
  • Description
    Mouse monoclonal [710-1E-94] to Alpha 2 Antiplasmin
  • Specificityab30994 is specific to human Alpha 2 Antiplasmin.
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, human Alpha 2 Antiplasmin.

Properties

Applications

Our Abpromise guarantee covers the use of ab30994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/50000.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 55 kDa. Dilution optimised using Chromogenic detection.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionSerine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Involvement in diseaseDefects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID) [MIM:262850]. APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis.
  • Sequence similaritiesBelongs to the serpin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • A2AP antibody
    • A2AP_HUMAN antibody
    • AAP antibody
    • Alpha 2 antiplasmin antibody
    • Alpha 2 antiplasmin pigment epithelium derived factor antibody
    • Alpha 2 AP antibody
    • ALPHA 2 PI antibody
    • Alpha 2 plasmin inhibitor antibody
    • Alpha 2 plasmin inhibitor deficiency antibody
    • Alpha-2-antiplasmin antibody
    • Alpha-2-AP antibody
    • Alpha-2-PI antibody
    • Alpha-2-plasmin inhibitor antibody
    • Antiplasmin deficiency antibody
    • API antibody
    • Plasmin inhibitor deficiency antibody
    • PLI antibody
    • Serine (or cysteine) peptidase inhibitor, clade F, member 2 antibody
    • Serine (Or cysteine) peptidase inhibitor, clade F, member 2, isoform CRA_c antibody
    • Serine (or cysteine) proteinase inhibitor, clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2 antibody
    • Serine (Or cysteine) proteinase inhibitor, clade F, member 2 antibody
    • Serine or cysteine peptidase inhibitor clade F member 2 antibody
    • Serpin F2 antibody
    • Serpin peptidase inhibitor clade F antibody
    • Serpin peptidase inhibitor, clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2 antibody
    • SERPINF2 antibody
    see all

Anti-Alpha 2 Antiplasmin antibody [710-1E-94] images

  • All lanes : Anti-Alpha 2 Antiplasmin antibody [710-1E-94] (ab30994) at 1/300 dilution

    Lane 1 : Purified alpha 2-antiplasmin
    Lane 2 : Whole tissue lysate prepared from human brain

    Lysates/proteins at 30 µg per lane.

    Secondary
    Horse anti-mouse polyclonal conjugated to HRP at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 55 kDa
    Observed band size : 55 kDa


    Exposure time : 5 minutes

    Image courtesy of Mrs Rachel Barker by Abreview.

    See Abreview

References for Anti-Alpha 2 Antiplasmin antibody [710-1E-94] (ab30994)

This product has been referenced in:
  • Li Q  et al. Plasmin plays an essential role in amplification of psoriasiform skin inflammation in mice. PLoS One 6:e16483 (2011). WB ; Human . Read more (PubMed: 21311769) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (brain)
Loading amount 30 µg
Specification brain
Gel Running Conditions Reduced Denaturing (4-20% Tris-HCl gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Rachel Barker

Verified customer

Submitted Oct 28 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA (pH8)
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Rachel Barker

Verified customer

Submitted Oct 28 2010

Thank you for your enquiry. The originator of the antibody noticed that while we are calling this antibody anti-PEDF, the target should actually be alpha 2 antiplasmin. Since the two proteins are slightly different at the N terminus, this could exp...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"