Anti-Alpha 2 Antiplasmin antibody (ab62770)

Overview

  • Product name
    Anti-Alpha 2 Antiplasmin antibody
    See all Alpha 2 Antiplasmin primary antibodies
  • Description
    Rabbit polyclonal to Alpha 2 Antiplasmin
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human alpha 2 antiplasmin.

  • Positive control
    • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab62770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 55 kDa.
ELISA 1/10000.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.
  • Tissue specificity
    Expressed by the liver and secreted in plasma.
  • Involvement in disease
    Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID) [MIM:262850]. APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis.
  • Sequence similarities
    Belongs to the serpin family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • A2AP antibody
    • A2AP_HUMAN antibody
    • AAP antibody
    • Alpha 2 antiplasmin antibody
    • Alpha 2 antiplasmin pigment epithelium derived factor antibody
    • Alpha 2 AP antibody
    • ALPHA 2 PI antibody
    • Alpha 2 plasmin inhibitor antibody
    • Alpha 2 plasmin inhibitor deficiency antibody
    • Alpha-2-antiplasmin antibody
    • Alpha-2-AP antibody
    • Alpha-2-PI antibody
    • Alpha-2-plasmin inhibitor antibody
    • Antiplasmin deficiency antibody
    • API antibody
    • Plasmin inhibitor deficiency antibody
    • PLI antibody
    • Serine (or cysteine) peptidase inhibitor, clade F, member 2 antibody
    • Serine (Or cysteine) peptidase inhibitor, clade F, member 2, isoform CRA_c antibody
    • Serine (or cysteine) proteinase inhibitor, clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2 antibody
    • Serine (Or cysteine) proteinase inhibitor, clade F, member 2 antibody
    • Serine or cysteine peptidase inhibitor clade F member 2 antibody
    • Serpin F2 antibody
    • Serpin peptidase inhibitor clade F antibody
    • Serpin peptidase inhibitor, clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2 antibody
    • SERPINF2 antibody
    see all

Images

  • ICC/IF image of ab62770 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab62770 at 5 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab62770 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (brain)
Loading amount
30 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (4-20% Tris-HCl gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Rachel Barker

Verified customer

Submitted Nov 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Cell (Brain)
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Type
Sandwich (Detection)
Username

Dr. Rachel Barker

Verified customer

Submitted Nov 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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