Recombinant Anti-Alpha 2 Antiplasmin antibody [EPSIS17] (ab150414)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPSIS17] to Alpha 2 Antiplasmin
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Alpha 2 Antiplasmin antibody [EPSIS17]
See all Alpha 2 Antiplasmin primary antibodies -
Description
Rabbit monoclonal [EPSIS17] to Alpha 2 Antiplasmin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Alpha 2 Antiplasmin aa 50-150. The exact sequence is proprietary.
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Positive control
- WB: K-562 whole cell lysates. IHC-P: Human liver tissue.
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General notes
This product has switched from a hybridoma to recombinant production method on 2nd August 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPSIS17 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000. Predicted molecular weight: 55 kDa.
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IHC-P |
1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/10000. Predicted molecular weight: 55 kDa. |
IHC-P
1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID) [MIM:262850]. APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis. -
Sequence similarities
Belongs to the serpin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5345 Human
- Omim: 613168 Human
- SwissProt: P08697 Human
- Unigene: 159509 Human
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Alternative names
- A2AP antibody
- A2AP_HUMAN antibody
- AAP antibody
see all
Images
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Anti-Alpha 2 Antiplasmin antibody [EPSIS17] (ab150414) at 1/10000 dilution + K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Alpha 2 Antiplasmin with ab150414 at 1/150 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab150414 has been referenced in 1 publication.
- Baker SK et al. Blood-derived plasminogen drives brain inflammation and plaque deposition in a mouse model of Alzheimer's disease. Proc Natl Acad Sci U S A 115:E9687-E9696 (2018). PubMed: 30254165