Overview

  • Product nameAnti-alpha 2 Catenin antibody
    See all alpha 2 Catenin primary antibodies
  • Description
    Rabbit polyclonal to alpha 2 Catenin
  • SpecificityAnti-alpha-N-Catenin recognizes alpha-N-catenin (102 kDa) by immunoblotting. Staining of the alpha-N-catenin band in immunoblotting is specifically inhibited with the alpha-N-catenin immunizing peptide (human, amino acids 171-186).
  • Tested applicationsSuitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide:

    DLANRFKEFGKKMVKL

    conjugated to KLH, corresponding to amino acids 171-186 of Human alpha 2 Catenin.

  • General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab11347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
WB
  • Application notesIHC-Fr: 1/500.
    WB: 1/1000 - 1/2000. Detects a band of approximately 102 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay function as a linker between cadherin adhesion receptors and the cytoskeleton to regulate cell-cell adhesion and differentiation in the nervous system. Regulates morphological plasticity of synapses and cerebellar and hippocampal lamination during development. Functions in the control of startle modulation.
    • Sequence similaritiesBelongs to the vinculin/alpha-catenin family.
    • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cell projection > axon. Recruited to the nucleus upon ZNF639 overexpression.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha catenin related protein antibody
      • Alpha N catenin antibody
      • Alpha N-catenin antibody
      • Alpha-catenin-related protein antibody
      • Cadherin associated protein related antibody
      • Cancer/testis antigen 114 antibody
      • CAP R antibody
      • CAPR antibody
      • Catenin (cadherin associated protein) alpha 2 antibody
      • Catenin alpha 2 antibody
      • Catenin alpha-2 antibody
      • CT114 antibody
      • CTNA2_HUMAN antibody
      • CTNN A2 antibody
      • CTNNA 2 antibody
      • CTNNA2 antibody
      • CTNR antibody
      • DKFZp686H02198 antibody
      see all

    Anti-alpha 2 Catenin antibody images

    • ab11347 staining alpha 2 Catenin (left) in Mouse cortical ventricular zone tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde in PBS overnight at 4°C, then saturated in 30% sucrose at 4°C (for at least 18 hours). Fixed and saturated brains were embedded in OCT compound and frozen rapidly with dry ice, and then 12µm cyrosections collected and stored at -80°C. Samples were incubated with primary antibody (1/500). Middle - non-phosphorylated active beta catenin, Right - merge. Beta catenin co-localizes with alpha catenin at the apical junctions rings.

    References for Anti-alpha 2 Catenin antibody (ab11347)

    This product has been referenced in:
    • Butko MT  et al. In vivo quantitative proteomics of somatosensory cortical synapses shows which protein levels are modulated by sensory deprivation. Proc Natl Acad Sci U S A 110:E726-35 (2013). WB ; Mouse . Read more (PubMed: 23382246) »
    • Thompson J  et al. Adherens junction breakdown in the periderm following cadmium administration in the chick embryo: distribution of cadherins and associated molecules. Reprod Toxicol 25:39-46 (2008). IHC-P ; Chicken . Read more (PubMed: 18031986) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"