The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Use at an assay dependent dilution. Predicted molecular weight: 163 kDa.
FunctionIs able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Tissue specificitySecreted in plasma.
Sequence similaritiesBelongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
Developmental stageContrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
References for Anti-alpha 2 Macroglobulin antibody (ab48555)
This product has been referenced in:
Liu Z et al. Tandem 18O stable isotope labeling for quantification of N-glycoproteome. J Proteome Res9:227-36 (2010).
Read more (PubMed: 19921957) »
Insenser M et al. Proteomic analysis of plasma in the polycystic ovary syndrome identifies novel markers involved in iron metabolism, acute-phase response, and inflammation. J Clin Endocrinol Metab95:3863-70 (2010).
Read more (PubMed: 20484478) »