Anti-alpha 2a Adrenergic Receptor antibody (ab45871)

Overview

  • Product nameAnti-alpha 2a Adrenergic Receptor antibody
    See all alpha 2a Adrenergic Receptor primary antibodies
  • Description
    Goat polyclonal to alpha 2a Adrenergic Receptor
  • Tested applicationsSuitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Dog, Human
  • Immunogen

    Synthetic peptide:

    C-TERRPNGLGPERS

    , corresponding to Internal sequence amino acids 246-258 of Human alpha 2a Adrenergic Receptor

  • Positive control
    • Human brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab45871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 19126537

Target

  • FunctionAlpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2A sub-subfamily.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADA2A_HUMAN antibody
    • ADRA2 antibody
    • ADRA2A antibody
    • ADRA2R antibody
    • ADRAR antibody
    • Adrenergic alpha 2A receptor antibody
    • Alpha 2 adrenergic receptor subtype C10 antibody
    • Alpha 2A adrenergic receptor antibody
    • Alpha 2AAR subtype C10 antibody
    • Alpha-2 adrenergic receptor subtype C10 antibody
    • alpha-2-adrenergic receptor, platelet type antibody
    • Alpha-2A adrenergic receptor antibody
    • Alpha-2A adrenoceptor antibody
    • Alpha-2A adrenoreceptor antibody
    • Alpha-2AAR antibody
    • ALPHA2AAR antibody
    • OTTHUMP00000058849 antibody
    • ZNF32 antibody
    see all

Anti-alpha 2a Adrenergic Receptor antibody images

  • ab45871 staining alpha 2a Adrenergic Receptor in mouse DRG neurons by Immunocytochemistry/ Immunofluorescence. The ganglia were collected from postnatal day 0–3 pups of C57 BL/6 mice and enzymatically dissociated with medium containing 0.25% trypsin at 37°C. Dissociated neurons were then plated onto glasscoverslips and maintained at 37°C in culture media. After drug treatment, the cultures were fixed with 4% paraformaldehyde for 10 min, washed in phosphate-buffered saline (PBS), permeabilized, and preblocked with PBS containing 10% normal donkey serum and 0.1% Triton X-100 for 2 h at room temperature. The cultures were then incubated overnight at 4 °C with the primary antibodies. A Biotin-conjugated anti-goat IgG and Cy3-conjugated streptavidin was used to detect alpha 2a adrenergic receptor.

References for Anti-alpha 2a Adrenergic Receptor antibody (ab45871)

This product has been referenced in:
  • Alba-Delgado C  et al. The function of alpha-2-adrenoceptors in the rat locus coeruleus is preserved in the chronic constriction injury model of neuropathic pain. Psychopharmacology (Berl) : (2011). WB, IHC-FrFl ; Rat . Read more (PubMed: 22038538) »
  • Tan M  et al. p38 MAPK and beta-arrestin 2 mediate functional interactions between endogenous micro-opioid and alpha2A-adrenergic receptors in neurons. J Biol Chem 284:6270-81 (2009). ICC/IF ; Mouse . Read more (PubMed: 19126537) »

See all 2 Publications for this product

Product Wall

The blocking peptide for ab85570 is ab102061.

For ab45871, ab54291 can be used.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"