Recombinant Anti-ADRA2B antibody [EPR9623] (ab151727)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9623] to ADRA2B
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ADRA2B antibody [EPR9623]
See all ADRA2B primary antibodies -
Description
Rabbit monoclonal [EPR9623] to ADRA2B -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
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Positive control
- Human fetal brain, MCF7, HepG2 and 293T cell lysates; 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9623 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 50 kDa.
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Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 50 kDa. |
Target
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Relevance
Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Alpha 2B adrenergic receptor subtype was observed to associate with eIF-2B, a guanine nucleotide exchange protein that functions in regulation of translation. A polymorphic variant of the alpha2B subtype, which lacks 3 glutamic acids from a glutamic acid repeat element, was identified to have decreased G protein-coupled receptor kinase-mediated phosphorylation and desensitization; this polymorphic form is also associated with reduced basal metabolic rate in obese subjects and may therefore contribute to the pathogenesis of obesity. Alpha 2B adrenergic receptor gene contains no introns in either its coding or untranslated sequences. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 151 Human
- Entrez Gene: 11552 Mouse
- Entrez Gene: 24174 Rat
- Omim: 104260 Human
- SwissProt: P18089 Human
- SwissProt: P30545 Mouse
- SwissProt: P19328 Rat
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Alternative names
- ADRA2B antibody
- ADRA2RL1 antibody
- ADRARL1 antibody
see all
Images
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All lanes : Anti-ADRA2B antibody [EPR9623] (ab151727) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 50 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab151727 has been referenced in 11 publications.
- Wang J et al. Norepinephrine exposure restrains endometrial decidualization during early pregnancy. J Endocrinol 248:277-288 (2021). PubMed: 33434145
- Kokoz YM et al. Upregulation of a2-adrenoceptor synthesis in SHR cardiomyocytes: Recompense without sense - Increased amounts, impaired commands. Arch Biochem Biophys 674:108109 (2019). PubMed: 31541620
- Zheng F et al. P2Y12 deficiency in mouse impairs noradrenergic system in brain, and alters anxiety-like neurobehavior and memory. Genes Brain Behav 18:e12458 (2019). PubMed: 29341465
- Maltsev AV et al. a2-Adrenoceptor signaling in cardiomyocytes of spontaneously hypertensive rats starts to impair already at early age. Biochem Biophys Res Commun 512:908-913 (2019). PubMed: 30929926
- Tyurin-Kuzmin PA et al. Flow cytometry analysis of adrenoceptors expression in human adipose-derived mesenchymal stem/stromal cells. Sci Data 5:180196 (2018). PubMed: 30277480