Recombinant
RabMAb

Anti-alpha 2b Adrenergic Receptor antibody [EPR9623] (ab151727)

Overview

  • Product name
    Anti-alpha 2b Adrenergic Receptor antibody [EPR9623]
    See all alpha 2b Adrenergic Receptor primary antibodies
  • Description
    Rabbit monoclonal [EPR9623] to alpha 2b Adrenergic Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human alpha 2b Adrenergic Receptor aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P18089
    (Peptide available as ab177733)

  • Positive control
    • Human fetal brain, MCF7, HepG2 and 293T cell lysates; 293T cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 50 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Relevance
      Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Alpha 2B adrenergic receptor subtype was observed to associate with eIF-2B, a guanine nucleotide exchange protein that functions in regulation of translation. A polymorphic variant of the alpha2B subtype, which lacks 3 glutamic acids from a glutamic acid repeat element, was identified to have decreased G protein-coupled receptor kinase-mediated phosphorylation and desensitization; this polymorphic form is also associated with reduced basal metabolic rate in obese subjects and may therefore contribute to the pathogenesis of obesity. Alpha 2B adrenergic receptor gene contains no introns in either its coding or untranslated sequences.
    • Cellular localization
      Cell Membrane
    • Database links
    • Alternative names
      • ADRA2B antibody
      • ADRA2RL1 antibody
      • ADRARL1 antibody
      • Alpha 2 adrenergic receptor subtype C2 antibody
      • Alpha 2B adrenoceptor antibody
      • Alpha 2B adrenoreceptor antibody
      • alpha 2BAR antibody
      • ALPHA2BAR antibody
      see all

    Images

    • All lanes : Anti-alpha 2b Adrenergic Receptor antibody [EPR9623] (ab151727) at 1/10000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 50 kDa

    • Flow cytometric analysis of 293T cells labeling alpha 2b Adrenergic Receptor with ab151727 at 1/10 dilution (red) compared to a nonspecific control antibody (green).

    References

    This product has been referenced in:
    • Tyurin-Kuzmin PA  et al. Activation of ß-adrenergic receptors is required for elevated a1A-adrenoreceptors expression and signaling in mesenchymal stromal cells. Sci Rep 6:32835 (2016). Flow Cyt . Read more (PubMed: 27596381) »
    • Xiao L  et al. Renal Denervation Prevents Immune Cell Activation and Renal Inflammation in Angiotensin II-Induced Hypertension. Circ Res 117:547-57 (2015). Read more (PubMed: 26156232) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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