The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 50 kDa.
Use at an assay dependent concentration.
The alpha 2C Adrenergic Receptor controls the release of neurotransmitter from central adrenergic neurons and from sympathetic nerves in the heart. This receptor also plays a role in cognitive and behavioral function. Two variants are produced by alternative splicing. Alpha 2C Adrenergic Receptor has been reported extensively in the brain. Blood, corpus cavermosum, kidney, and heart tissues have also been reported to express the protein.
4% formaldehyde-fixed SK-N-BE cells labeling alpha 2C Adrenergic Receptor using ab167433 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/100 dilution incubated for 1 hour at room temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).
Western blot - Anti-alpha 2C Adrenergic Receptor antibody [N330A/80] (ab167433)
Anti-alpha 2C Adrenergic Receptor antibody [N330A/80] (ab167433) at 1/200 dilution + Monkey COS cells transfected with HA-tagged alpha 2C Adrenergic Receptor
Secondary Goat anti-mouse IgG at 1/1000 dilution
Predicted band size: 50 kDa
Exposure time: 6 minutes
Membrane was blocked with 2% BSA and 2% Skim Milk in 1X TBST.
This product has been referenced in:
Albee LJ et al. a1-Adrenergic Receptors Function Within Hetero-Oligomeric Complexes With Atypical Chemokine Receptor 3 and Chemokine (C-X-C motif) Receptor 4 in Vascular Smooth Muscle Cells. J Am Heart Assoc6:N/A (2017).
Read more (PubMed: 28862946) »
Tripathi A et al. Heteromerization of chemokine (C-X-C motif) receptor 4 with a1A/B-adrenergic receptors controls a1-adrenergic receptor function. Proc Natl Acad Sci U S A112:E1659-68 (2015).
Read more (PubMed: 25775528) »