Anti-alpha 2u-globulin antibody [33B10] (ab27161)

Overview

  • Product name
    Anti-alpha 2u-globulin antibody [33B10]
    See all alpha 2u-globulin primary antibodies
  • Description
    Mouse monoclonal [33B10] to alpha 2u-globulin
  • Tested applications
    Suitable for: Sandwich ELISA, ELISA, WBmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Full length native protein (purified) corresponding to Rat alpha 2u-globulin .

  • General notes

    This antibody, in combination with ab27160, is well suited to be used in sandwich ELISA for detection of pico gramme amounts of Alpha 2 microglobulin.

Properties

Applications

Our Abpromise guarantee covers the use of ab27161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [1D11] to alpha 2u-globulin (ab27160).
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 20 kDa.

Target

  • Relevance
    Alpha 2 microglobulin is part of the lipocalin family. There are many different isoforms which exhibit extensive homology. It is a major urinary protein in male rats and is synthesised and secreted by hepatic parenchymal cells. Alpha 2 microglobulin is also known as MUP (major urinary protein) and is a very important allergen because it constitutes approximately 30% of the total protein content excreted in the urine. Rats are the most frequently used laboratory animals and allergy to them constitutes an occupational disease. Approximately 20 % of the personal engaged in work with laboratory animals have acquired symptoms of allergy.
  • Cellular localization
    Cytoplasmic and Secreted
  • Database links
  • Alternative names
    • Allergen Rat n 1 antibody
    • Alpha (2) euglobulin antibody
    • Alpha 2u globulin antibody
    • Alpha2mu globulin antibody
    • Alpha2u antibody
    • Major urinary protein antibody
    • MUP antibody
    • PGCL1 antibody
    • Rat n1 antibody
    see all

References for Anti-alpha 2u-globulin antibody [33B10] (ab27161)

ab27161 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidney)
Antigen retrieval step
None
Permeabilization
No
Specification
Kidney
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Username

Ms. Katie Belsham

Verified customer

Submitted Nov 04 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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