Anti-alpha 3 Sodium Potassium ATPase antibody (ab150287)

Overview

  • Product name
    Anti-alpha 3 Sodium Potassium ATPase antibody
    See all alpha 3 Sodium Potassium ATPase primary antibodies
  • Description
    Rabbit polyclonal to alpha 3 Sodium Potassium ATPase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Rabbit, Horse, Cow, Dog, Human, Pig, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Rat alpha 3 Sodium Potassium ATPase.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Rat Brain; Rat Cerebellum; Rat Cortex; Mouse Brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab150287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 111 kDa).

Target

  • Function
    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • Involvement in disease
    Dystonia 12
    Alternating hemiplegia of childhood 2
    Cerebellar ataxia, areflexia, pes cavus, optic atrophy, and sensorineural hearing loss
  • Sequence similarities
    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHC2 antibody
    • Alpha(III) antibody
    • AT1A3_HUMAN antibody
    • Atp1a3 antibody
    • ATPase Na+/K+ transporting alpha 3 polypeptide antibody
    • DYT 12 antibody
    • DYT12 antibody
    • MGC13276 antibody
    • Na(+)/K(+) ATPase alpha(III) subunit antibody
    • Na(+)/K(+) ATPase alpha-3 subunit antibody
    • Na+/K+ ATPase 3 antibody
    • Na+/K+ ATPase alpha 3 subunit antibody
    • RDP antibody
    • Sodium potassium ATPase alpha 3 polypeptide antibody
    • Sodium pump 3 antibody
    • Sodium pump subunit alpha-3 antibody
    • Sodium/potassium transporting ATPase alpha 3 chain antibody
    • Sodium/potassium-transporting ATPase subunit alpha-3 antibody
    see all

Images

  • All lanes : Anti-alpha 3 Sodium Potassium ATPase antibody (ab150287) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Cerebellum (Rat) Tissue Lysate
    Lane 3 : Cortex (Rat) Tissue Lysate
    Lane 4 : Kidney (Rat) Tissue Lysate
    Lane 5 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 111 kDa
    Observed band size: 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 120 kDa, 55 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    Please note that lane 4 (Rat Kidney tissue lysate) represents a Negative control for alpha 3 Sodium Potassium ATPase. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab150287 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab150287 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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