Anti-alpha 5 Defensin antibody [8C8] (ab90802)


  • Product nameAnti-alpha 5 Defensin antibody [8C8]
    See all alpha 5 Defensin primary antibodies
  • Description
    Mouse monoclonal [8C8] to alpha 5 Defensin
  • Specificityab90802 recognizes the propeptide and the partially processed forms (aa 36-94 and 56-94), but it hardly recognizes mature peptide. No cross reactivity with HD6, lysozyme, or sPLA2 has be detected.
  • Tested applicationsSuitable for: WB, IHC-Fr, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment: ESLQERADEA TTQKQSGEDN QDLAISFAGN GLSALRTSGS QARATCYCRT GRCATRESLS GVCEISGRLY RLCCR, corresponding to amino acids 20-94 of Human alpha 5 Defensin purified from baculovirus infected Hi5 insect cells (UniProt Q01523).


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesab90802 was filtered through a 0.2 µm filter.
  • ClonalityMonoclonal
  • Clone number8C8
  • IsotypeIgG2b
  • Research areas


Our Abpromise guarantee covers the use of ab90802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Predicted molecular weight: 10 kDa.
IHC-Fr 1/50.
ELISA Use at an assay dependent concentration.
IHC-P 1/50.


  • FunctionHas antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing.
  • Tissue specificityPaneth cells of the small intestine (at protein level).
  • Sequence similaritiesBelongs to the alpha-defensin family.
  • Post-translational
  • Cellular localizationSecreted. Cytoplasmic vesicle > secretory vesicle. Peptides HD5(20-94), HD5(23-94) and HD5(29-94) are found within tissues, HD5(20-94) being the predominant intracellular form. Peptides HD5(56-94) and HD5(63-94) are found in the extracellular milieu, HD5(63-94) being the most abundant form.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha 5 antibody
    • DEF5 antibody
    • DEF5_HUMAN antibody
    • Defa antibody
    • Defa29 antibody
    • DEFA5 antibody
    • Defcr29 antibody
    • Defcr5 antibody
    • Defensin 5 antibody
    • Defensin alpha 5 antibody
    • Defensin alpha 5 Paneth cell specific antibody
    • Defensin alpha 5 preproprotein antibody
    • Defensin antibody
    • Enteric defensin antibody
    • HD 5 antibody
    • HD5 antibody
    • HD5(20-94) antibody
    • HD5(63-94) antibody
    • MGC129728 antibody
    • RD 5 antibody
    see all

Anti-alpha 5 Defensin antibody [8C8] images

  • ab90802 at 1/50 dilution staining alpha 5 Defensin in human Ileus tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). 

References for Anti-alpha 5 Defensin antibody [8C8] (ab90802)

This product has been referenced in:
  • Spencer JD  et al. Human alpha defensin 5 expression in the human kidney and urinary tract. PLoS One 7:e31712 (2012). IHC ; Human . Read more (PubMed: 22359618) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rhesus monkey Tissue sections (Ileum)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Specification Ileum
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde

Dr. B Pahar

Verified customer

Submitted Jan 29 2016

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience th...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Ileum human)
Specification Ileum human
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Antigen retrieval buffer pH 6.0
Permeabilization No
Blocking step 70% Ethanol with H2O2 as blocking agent for 10 minute(s) · Concentration: 0.2% · Temperature: 25°C

Dr. Ilka Werner-Martini

Verified customer

Submitted Jun 30 2011