Anti-alpha 5 Defensin antibody [8C8] (ab90802)

Overview

  • Product name
    Anti-alpha 5 Defensin antibody [8C8]
    See all alpha 5 Defensin primary antibodies
  • Description
    Mouse monoclonal [8C8] to alpha 5 Defensin
  • Host species
    Mouse
  • Specificity
    ab90802 recognizes the propeptide and the partially processed forms (aa 36-94 and 56-94), but it hardly recognizes mature peptide. No cross reactivity with HD6, lysozyme, or sPLA2 has be detected.
  • Tested applications
    Suitable for: WB, IHC-Fr, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment: ESLQERADEA TTQKQSGEDN QDLAISFAGN GLSALRTSGS QARATCYCRT GRCATRESLS GVCEISGRLY RLCCR, corresponding to amino acids 20-94 of Human alpha 5 Defensin purified from baculovirus infected Hi5 insect cells (UniProt Q01523).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab90802 was filtered through a 0.2 µm filter.
  • Clonality
    Monoclonal
  • Clone number
    8C8
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Predicted molecular weight: 10 kDa.
IHC-Fr 1/50.
ELISA Use at an assay dependent concentration.
IHC-P 1/50.

Target

  • Function
    Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing.
  • Tissue specificity
    Paneth cells of the small intestine (at protein level).
  • Sequence similarities
    Belongs to the alpha-defensin family.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Secreted. Cytoplasmic vesicle > secretory vesicle. Peptides HD5(20-94), HD5(23-94) and HD5(29-94) are found within tissues, HD5(20-94) being the predominant intracellular form. Peptides HD5(56-94) and HD5(63-94) are found in the extracellular milieu, HD5(63-94) being the most abundant form.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha 5 antibody
    • DEF5 antibody
    • DEF5_HUMAN antibody
    • Defa antibody
    • Defa29 antibody
    • DEFA5 antibody
    • Defcr29 antibody
    • Defcr5 antibody
    • Defensin 5 antibody
    • Defensin alpha 5 antibody
    • Defensin alpha 5 Paneth cell specific antibody
    • Defensin alpha 5 preproprotein antibody
    • Defensin antibody
    • Enteric defensin antibody
    • HD 5 antibody
    • HD5 antibody
    • HD5(20-94) antibody
    • HD5(63-94) antibody
    • MGC129728 antibody
    • RD 5 antibody
    see all

Images

  • ab90802 at 1/50 dilution staining alpha 5 Defensin in human Ileus tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). 

References

This product has been referenced in:
  • Spencer JD  et al. Human alpha defensin 5 expression in the human kidney and urinary tract. PLoS One 7:e31712 (2012). IHC ; Human . Read more (PubMed: 22359618) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Ileum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Specification
Ileum
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Dr. Bapi Pahar

Verified customer

Submitted Jan 29 2016

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience th...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Ileum human)
Specification
Ileum human
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Antigen retrieval buffer pH 6.0
Permeabilization
No
Blocking step
70% Ethanol with H2O2 as blocking agent for 10 minute(s) · Concentration: 0.2% · Temperature: 25°C
Username

Dr. Ilka Werner-Martini

Verified customer

Submitted Jun 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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