Recombinant
RabMAb

Anti-alpha 5 Defensin antibody [EPR14309(B)] (ab180515)

Overview

  • Product name
    Anti-alpha 5 Defensin antibody [EPR14309(B)]
    See all alpha 5 Defensin primary antibodies
  • Description
    Rabbit monoclonal [EPR14309(B)] to alpha 5 Defensin
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human alpha 5 Defensin aa 1 to the C-terminus (internal sequence). The exact sequence is proprietary.
    Database link: Q01523

  • Positive control
    • Human small intestine - paraffin embedded tissue and tissue lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR14309(B)
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab180515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 10 kDa.
IHC-P 1/4000.

Target

  • Function
    Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing.
  • Tissue specificity
    Paneth cells of the small intestine (at protein level).
  • Sequence similarities
    Belongs to the alpha-defensin family.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Secreted. Cytoplasmic vesicle > secretory vesicle. Peptides HD5(20-94), HD5(23-94) and HD5(29-94) are found within tissues, HD5(20-94) being the predominant intracellular form. Peptides HD5(56-94) and HD5(63-94) are found in the extracellular milieu, HD5(63-94) being the most abundant form.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha 5 antibody
    • DEF5 antibody
    • DEF5_HUMAN antibody
    • Defa antibody
    • Defa29 antibody
    • DEFA5 antibody
    • Defcr29 antibody
    • Defcr5 antibody
    • Defensin 5 antibody
    • Defensin alpha 5 antibody
    • Defensin alpha 5 Paneth cell specific antibody
    • Defensin alpha 5 preproprotein antibody
    • Defensin antibody
    • Enteric defensin antibody
    • HD 5 antibody
    • HD5 antibody
    • HD5(20-94) antibody
    • HD5(63-94) antibody
    • MGC129728 antibody
    • RD 5 antibody
    see all

Images

  • Anti-alpha 5 Defensin antibody [EPR14309(B)] (ab180515) at 1/10000 dilution + Human small intestine lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size : 10 kDa
  • Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling alpha 5 Defensin with ab180515 at 1/4000 dilution. Anti-rabbit HRP secondary antibody was used and counter staining with Hematoxylin performed. 

References

ab180515 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Duodenum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC2 (Low pH)
Permeabilization
No
Specification
Duodenum
Fixative
Paraformaldehyde
Username

Lina Li

Verified customer

Submitted May 31 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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