Anti-alpha Actinin antibody [0.T.02] (ab18061)

  Western blot - alpha Actinin antibody [0.T.02] (ab18061)

Anti-alpha Actinin antibody [0.T.02] (ab18061) at 1/2000 dilution + Human pancreatic cancer cell line - whole cell lysate at 20 µg

Secondary
HRP conjugated goat anti-mouse antibody at 1/2000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 103 kDa
Observed band size : 103 kDa


Exposure time : 1 minute

This image is courtesy of an anonymous Abreview

See Abreview

  Immunocytochemistry/ Immunofluorescence-alpha Actinin antibody [0.T.02](ab18061)

ICC/IF image of ab18061 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18061, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.