Anti-alpha Actinin antibody [EP2528Y] (ab81265)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-alpha Actinin antibody [EP2528Y]
See all alpha Actinin primary antibodies -
DescriptionRabbit monoclonal [EP2528Y] to alpha Actinin
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human alpha Actinin aa 750-850 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: NIH 3T3, C2C12, C6 and A673 cell lysates and human skeletal muscle and human heart tissue lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEP2528Y
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab81265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa). |
Target
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FunctionF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
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Sequence similaritiesBelongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.
- Information by UniProt
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Database links
- Entrez Gene: 87 Human
- Entrez Gene: 109711 Mouse
- Entrez Gene: 81634 Rat
- Omim: 102575 Human
- SwissProt: P12814 Human
- SwissProt: Q7TPR4 Mouse
- SwissProt: Q9Z1P2 Rat
- Unigene: 509765 Human
see all -
Alternative names
- actinin 1 smooth muscle antibody
- Actinin alpha 1 antibody
- actinin, alpha 1 antibody
see all
Images
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![Western blot - Anti-alpha Actinin antibody [EP2528Y] (ab81265)](http://a.abcam.com/ps/products/81/ab81265/Images/ab81265-299870-Ab81265KOWBDOC.jpg)
Western blot - Anti-alpha Actinin antibody [EP2528Y] (ab81265)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Alpha Actinin knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: NIH 3T3 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab81265 observed at 103 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab81265 was shown to recognize alpha Actinin in wild-type HAP1 cells as signal was lost at the expected MW in alpha Actinin knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and alpha Actinin knockout samples were subjected to SDS-PAGE. ab81265 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-alpha Actinin antibody [EP2528Y] (ab81265)](http://a.abcam.com/ps/products/81/ab81265/Images/ab81265-3-ab81265WB1.jpg)
Western blot - Anti-alpha Actinin antibody [EP2528Y] (ab81265)All lanes : Anti-alpha Actinin antibody [EP2528Y] (ab81265) at 1/1000 dilution (purified)
Lane 1 : NIH/3T3 whole cell lysate
Lane 2 : C2C12 whole cell lysate
Lane 3 : C6 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDa
Blocking and dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-alpha Actinin antibody [EP2528Y] (ab81265)](http://a.abcam.com/ps/products/81/ab81265/Images/ab81265-4-ab81265WB2.jpg)
Western blot - Anti-alpha Actinin antibody [EP2528Y] (ab81265)Anti-alpha Actinin antibody [EP2528Y] (ab81265) at 1/1000 dilution (purified) + A673 whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDa
Blocking and dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-alpha Actinin antibody [EP2528Y] (ab81265)](http://a.abcam.com/ps/products/81/ab81265/Images/alpha-Actinin-Primary-antibodies-ab81265-1.jpg)
Western blot - Anti-alpha Actinin antibody [EP2528Y] (ab81265)All lanes : Anti-alpha Actinin antibody [EP2528Y] (ab81265) at 1/1000 dilution (unpurified)
Lane 1 : NIH 3T3 cell lysate
Lane 2 : human skeletal muscle tissue lysate
Lane 3 : human heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 103 kDa
Observed band size: 103 kDa
Protocols
References
ab81265 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"