Validated using a knockout cell line
Recombinant
RabMAb

Anti-alpha Actinin antibody [EP2528Y] (ab81265)

Overview

  • Product name
    Anti-alpha Actinin antibody [EP2528Y]
    See all alpha Actinin primary antibodies
  • Description
    Rabbit monoclonal [EP2528Y] to alpha Actinin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human alpha Actinin aa 750-850 (C terminal). The exact sequence is proprietary.

  • Positive control
    • WB: NIH 3T3, C2C12, C6 and A673 cell lysates and human skeletal muscle and human heart tissue lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab81265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
    • Sequence similarities
      Belongs to the alpha-actinin family.
      Contains 1 actin-binding domain.
      Contains 2 CH (calponin-homology) domains.
      Contains 2 EF-hand domains.
      Contains 4 spectrin repeats.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.
    • Information by UniProt
    • Database links
    • Alternative names
      • actinin 1 smooth muscle antibody
      • Actinin alpha 1 antibody
      • actinin, alpha 1 antibody
      • ACTN 1 antibody
      • Actn1 antibody
      • ACTN1_HUMAN antibody
      • Alpha Actinin 1 antibody
      • Alpha actinin cytoskeletal isoform antibody
      • Alpha-actinin cytoskeletal isoform antibody
      • Alpha-actinin-1 antibody
      • BDPLT15 antibody
      • F actin cross linking protein antibody
      • F-actin cross-linking protein antibody
      • FLJ40884 antibody
      • FLJ54432 antibody
      • Non muscle alpha actinin 1 antibody
      • Non-muscle alpha-actinin-1 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Alpha Actinin knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: NIH 3T3 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab81265 observed at 103 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab81265 was shown to recognize alpha Actinin in wild-type HAP1 cells as signal was lost at the expected MW in alpha Actinin knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and alpha Actinin knockout samples were subjected to SDS-PAGE. ab81265 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-alpha Actinin antibody [EP2528Y] (ab81265) at 1/1000 dilution (purified)

      Lane 1 : NIH/3T3 whole cell lysate
      Lane 2 : C2C12 whole cell lysate
      Lane 3 : C6 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 103 kDa
      Observed band size: 103 kDa



      Blocking and dilution buffer: 5% NFDM/TBST
    • Anti-alpha Actinin antibody [EP2528Y] (ab81265) at 1/1000 dilution (purified) + A673 whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 103 kDa
      Observed band size: 103 kDa



      Blocking and dilution buffer: 5% NFDM/TBST
    • All lanes : Anti-alpha Actinin antibody [EP2528Y] (ab81265) at 1/1000 dilution (unpurified)

      Lane 1 : NIH 3T3 cell lysate
      Lane 2 : human skeletal muscle tissue lysate
      Lane 3 : human heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 103 kDa
      Observed band size: 103 kDa

    References

    ab81265 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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