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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Crosslinking
Anti-alpha Actinin antibody [MAC 276] (ab50599)
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- Secondary antibodies
- Isotype controls
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab50599? Please let us know so that we can cite the reference in this datasheet
ab50599 has been referenced in 2 publications.
- Lakey A et al. Identification and localization of high molecular weight proteins in insect flight and leg muscle. EMBO J 9:3459-67 (1990). PubMed: 2209553
- Kreisköther N et al. Drosophila Rolling pebbles colocalises and putatively interacts with alpha-Actinin and the Sls isoform Zormin in the Z-discs of the sarcomere and with Dumbfounded/Kirre, alpha-Actinin and Zormin in the terminal Z-discs. J Muscle Res Cell Motil : (2006). PubMed: 16625315
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
](/ps/datasheet/images/50/ab50599/alpha-Actinin-Primary-antibodies-ab50599-3.jpg)
Immunocytochemistry/ Immunofluorescence-alpha Actinin antibody [MAC 276](ab50599)
ICC/IF image of ab68826 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab50599 5µg/ml overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
](/ps/datasheet/images/50/ab50599/alpha-Actinin-Primary-antibodies-ab50599-4.jpg)
Flow Cytometry-alpha Actinin antibody [MAC 276](ab50599)
Overlay histogram showing HeLa cells stained with ab50599 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab50599, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (
![Western blot - Anti-alpha Actinin antibody [MAC 276] (ab50599)](/ps/datasheet/images/50/ab50599/alpha-Actinin-Primary-antibodies-ab50599-7.jpg)
Western blot - Anti-alpha Actinin antibody [MAC 276] (ab50599)
All lanes : Anti-alpha Actinin antibody [MAC 276] (ab50599) at 1 µg/ml
Lane 1 :
Lane 2 : Heart (Rat) Tissue Lysate
Lane 3 : Skeletal Muscle (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 103 kDa
Observed band size : 103 kDa
Exposure time : 8 minutes
![alpha Actinin antibody [MAC 276] for ICC/IF in Human (50599)](/ps/datasheet/images/50/ab50599/alpha-Actinin-Primary-antibodies-ab50599-2.jpg)
alpha Actinin antibody [MAC 276] for ICC/IF in Human (50599)
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