alpha Actinin antibody [MAC 276] (ab50599)

Product Name 

alpha Actinin antibody [MAC 276]

Product type 

Primary antibodies

Description 

Rat monoclonal [MAC 276] to alpha Actinin

Immunogen 

Flight muscle extract from Lethocerus indicus (Waterbug)

Reacts with 
(species key)

Hu, Rat, Chk, Dm, LtInd

Specificity 

Antibody reacts with a-actinin in Lethocerus and Drosophila flight and non-flight muscle. Antibody also reacts with rat skeletal muscle, human cardiac muscle, chicken gizzard muscle and human fibroblasts.

Tested applications 
(see key)

EM, ICC/IF

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Electron Microscopy: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic, colocalizes with MYOZ2 and PPP3CA at the Z line of heart and skeletal muscle.

Research areas 

Developmental Biology >> Lineage specification >> Mesoderm
Stem Cells >> Lineage Markers >> Mesoderm
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Crosslinking

Relevance 

Alpha Actinin is an actin binding protein present in both muscle and non muscle cells. It has a molecular weight of 100,000 daltons and forms dimers in solution. In normal skeletal muscle alpha actinin is associated with the z discs that define muscle sarcomeres. In smooth muscle alpha actinin has been detected in dense bodies and plaques characteristic of the tissue. Immunofluorescent labeling of a large variety of cells using antibody to alpha actinin reveals extensive association of the proteins with actin containing stress fibers and in particular with their membrane bound termini.

Database links 

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

MAC 276

Isotype 

IgG2a

Purity 

Protein G purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for alpha Actinin antibody [MAC 276] (ab50599)

  This product has been used in: (two most recent references)

Kreiskther N et al. Drosophila Rolling pebbles colocalises and putatively interacts with alpha-Actinin and the Sls isoform Zormin in the Z-discs of the sarcomere and with Dumbfounded/Kirre, alpha-Actinin and Zormin in the terminal Z-discs. J Muscle Res Cell Motil : (2006). PubMed: 16625315

Lakey A et al. Identification and localization of high molecular weight proteins in insect flight and leg muscle. EMBO J 9:3459-67 (1990). PubMed: 2209553
If you publish research using ab50599 please let us know so that we can cite the reference on this datasheet.

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