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Products:Signal Transduction >> Adapters >> Transmembrane
Overview
Product name
Anti-alpha Adaptin antibody [100/2]
See all alpha Adaptin products (4) ...
Description
Mouse monoclonal [100/2] to alpha Adaptin
Specificity
Reacts in bovine and rat brain preparations, with the 105 and 110 alpha-subunits of adaptor complex AP2. The antibody reacts with polypeptides of approximately 100 kDa in bovine liver, human heart fibroblasts, and Madin-Darby bovine kidney cultured cells (MDBK), but not with any components in the 110-115 kDa range from these sources or from rat pheochromocytoma cultured cells (PC12), neuroblastoma or astrocytes. The antibody stains the intact alpha adaptor, as well as the 37 and 40 kDa fragments, but not the 63-66 kDa group of alpha fragments obtained by trypsin cleavage of the alpha subunits molecules.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Rat, Cow, Human
Immunogen
AP2 polypeptides from bovine brain.
Positive control
WB: rat brain lysate, bovine liver, human heart fibroblasts, Madin-Darby bovine kidney cultured cells
General notes
Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
100/2
Isotype
IgG2a
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab11326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
WB
Application notes
ICC/IF: Use at an assay dependent dilution (see Domin et al).
WB: 1/200. Predicted molecular weight: 100 kDa.
May be unsuitable for IHC-Fr with PFA fixation (Customer feedback, see Abreviews).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Clathrin mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down regulated. During formation of clathrin coated membranes, clathrin co assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression.
Cellular localization
Cytoplasmic
Alternative names
- 100 kDa coated vesicle protein A antibody
- 100 kDa coated vesicle protein C antibody
- adaptin alpha A antibody
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Database links
- Entrez Gene: 160 Human
- Entrez Gene: 161 Human
- Entrez Gene: 308578 Rat
- Entrez Gene: 81637 Rat
- Omim: 601026 Human
- Omim: 607242 Human
- SwissProt: Q1JPJ7 Cow
- SwissProt: O94973 Human
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References for Anti-alpha Adaptin antibody [100/2] (ab11326)
This product has been referenced in:
- Swigut Tet al. Cooperative interactions of simian immunodeficiency virus Nef, AP-2, and CD3-zeta mediate the selective induction of T-cell receptor-CD3 endocytosis. J Virol 77:8116-26 (2003).Read more (PubMed: 12829850) »
- Olusanya Oet al. Phosphorylation of threonine 156 of the mu2 subunit of the AP2 complex is essential for endocytosis in vitro and in vivo. Curr Biol 11:896-900 (2001).Read more (PubMed: 11516654) »
See all 7 publications for this product
Publishing research using ab11326? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"