![Immunocytochemistry/ Immunofluorescence - alpha Adaptin antibody [AC1-M11] (ab2807) image](#ab2807_1.jpg)
![Immunocytochemistry - alpha Adaptin antibody [AC1-M11] (ab2807) image](#ab2807_2.jpg)
![Western blot - alpha Adaptin antibody [AC1-M11] (ab2807) image](#alpha-Adaptin-Primary-antibodies-ab2807-2.jpg)
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Products:Signal Transduction >> Adapters >> Transmembrane
Overview
Product name
Anti-alpha Adaptin antibody [AC1-M11]
See all alpha Adaptin products (4) ...
Description
Mouse monoclonal [AC1-M11] to alpha Adaptin
Specificity
Detects alpha Adaptin. This antibody detects the products of both alpha-adaptin genes, alpha A and alpha C, as well as an alternatively spliced isoform of alpha A found in neurons. By Western blot, this antibody recognizes an ~100 kDa doublet representing the two isoforms of alpha-adaptin from pig brain extract. Immunofluorescence staining of alpha-adaptin in transfected COS cells yields distinct punctate dots on the plasma membrane.
Tested applications
IP, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Cow, Human, Pig
Predicted to work with
Non Human Primates, Amphibians
Immunogen
Purified bovine brain adaptor complexes.
Positive control
pig brain lysate and transfected COS cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
AC1-M11
Isotype
IgG2a
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
WB: 1/100Detects a band of approximately 100 kDa.(In pig brain extract detects a doublet of approximately 100 kDa representing the two isoforms of alpha-adaptin.)
ICC/IF: 1/100
Target
Relevance
Clathrin mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down regulated. During formation of clathrin coated membranes, clathrin co assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression.
Cellular localization
Cytoplasmic
Alternative names
- 100 kDa coated vesicle protein A antibody
- 100 kDa coated vesicle protein C antibody
- adaptin alpha A antibody
see all
Database links
- Entrez Gene: 423102 Chicken
- Entrez Gene: 160 Human
- Entrez Gene: 161 Human
- Entrez Gene: 11771 Mouse
- Entrez Gene: 11772 Mouse
- Entrez Gene: 308578 Rat
- Entrez Gene: 81637 Rat
- Omim: 601026 Human
see all
Anti-alpha Adaptin antibody [AC1-M11] images:
Immunocytochemistry/ Immunofluorescence - alpha Adaptin antibody [AC1-M11] (ab2807)
![Immunocytochemistry/ Immunofluorescence - alpha Adaptin antibody [AC1-M11] (ab2807)](/ps/datasheet/Images/2/ab2807/ab2807_1.jpg)
Immunolocalization of alpha-adaptin in NRK cells using ab2807.
Immunocytochemistry - alpha Adaptin antibody [AC1-M11] (ab2807)
![Immunocytochemistry - alpha Adaptin antibody [AC1-M11] (ab2807)](/ps/datasheet/Images/2/ab2807/ab2807_2.jpg)
Immunolocalization of alpha-adaptin in NRK cells using ab2807 (low power image of image1).
Western blot - alpha Adaptin antibody [AC1-M11] (ab2807)
![Western blot - alpha Adaptin antibody [AC1-M11] (ab2807)](/ps/datasheet/images/2/ab2807/alpha-Adaptin-Primary-antibodies-ab2807-2.jpg)
Anti-alpha Adaptin antibody [AC1-M11] (ab2807) at 1/500 dilution + Lysate was prepared from mouse neuroblastoma cells at 10 µg
Secondary
HRP-conjugated donkey monoclonal to mouse IgG at 1/1000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 seconds
This image is a courtesy of Anonymous Abreview
References for Anti-alpha Adaptin antibody [AC1-M11] (ab2807)
This product has been referenced in:
- Robinson MS 100-kD coated vesicle proteins: molecular heterogeneity and intracellular distribution studied with monoclonal antibodies. J Cell Biol 104:887-95 (1987).Read more (PubMed: 2881934) »
See 1 publication for this product
Publishing research using ab2807? Please let us know so that we can cite the reference in this datasheet
![alpha Adaptin antibody [AC1-M11] for WB in Mouse (2807)](/ps/datasheet/images/2/ab2807/alpha-Adaptin-Primary-antibodies-ab2807-1.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"