alpha Adaptin antibody [AP6] (ab2730)

Product Name 

alpha Adaptin antibody [AP6]

Product type 

Primary antibodies

Description 

Mouse monoclonal [AP6] to alpha Adaptin

Immunogen 

Purified adaptors

Reacts with 
(species key)

Hu, Ms, Rat, Cow, Hm, NHuPrm

Specificity 

Detects assembly polypeptide 2 (AP2) It recognizes the products of both alpha-adaptin genes, alpha A and alpha C as well as an alternatively spliced isoform of alpha A found in neurons.

Tested applications 
(see key)

BL, ICC/IF, IHC-FoFr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Immunofluorescence: use at 1:200
Western Blot 1:1,000
This antibody detects an ~100 kDa doublet representing the two isoforms of alpha-adaptin in rat brain, though alpha C is the dominant form seen.
Microinjection of cells with this antibody results in variable blockade of endocytosis.

Cellular localization 

Cytoplasmic

Research areas 

Signal Transduction >> Adapters >> Transmembrane

Relevance 

Clathrin mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down regulated. During formation of clathrin coated membranes, clathrin co assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

AP6

Isotype 

IgG1

Purity 

IgG fraction

Storage buffer 

PBS with 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for alpha Adaptin antibody [AP6] (ab2730)

  This product has been used in: (two most recent references)

Ezratty EJ et al. Clathrin mediates integrin endocytosis for focal adhesion disassembly in migrating cells. J Cell Biol 187:733-47 (2009). ICC/IF; Mouse. PubMed: 19951918

Pryor PR et al. Molecular basis for the sorting of the SNARE VAMP7 into endocytic clathrin-coated vesicles by the ArfGAP Hrb. Cell 134:817-27 (2008). ICC/IF; Rat. PubMed: 18775314

See all 5 publication references for this product.


If you publish research using ab2730 please let us know so that we can cite the reference on this datasheet.

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