alpha Adducin antibody [EP734Y] (ab40760)

Product Name 

alpha Adducin antibody [EP734Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP734Y] to alpha Adducin

Immunogen 

A synthetic peptide corresponding to residues in the C-term of human Adducin was used as immunogen.

Reacts with 

Hu, Ms

Does not react with 
(species key)

Rat

Tested applications 
(see key)

ICC/IF, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/250 - 1/500.
IHC-P: 1/250 - 1/500.

WB: 1/5000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).

Is unsuitable for FACS or IP.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa cell lysate, human lung (IHC-p).

Cellular localization 

Cytoplasm, cytoskeleton and cell membrane

Research areas 

Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin

Relevance 

Alpha adducin is a 80-120 kD member of the aldolase class II family and adducin subfamily. This protein has three isoforms that interact as heterodimers alpha/beta, and beta/gamma. The molecular weights for the various isoforms alpha=120 kD, beta=110kD; gamma=80kD. The alpha adducin protein is a ubiquitously expressed cytoskeletal protein that binds with high affinity to Ca²⁺/calmodulin and acts as a substrate for protein kinases A and C. This protein has been reported to promote the assembly of the spectrinactin network. Polymorphisms in alpha adducin are associated with cardiovascular disease and hypertension. Alpha adducin has been reported to interact with calmodulin. When phosphorylated by Rho-kinase, alpha adducin can regulate membrane ruffling and cell motility

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP734Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

See below for alpha Adducin antibody [EP734Y] images, references, products related to ab40760 and other tools.

alpha Adducin antibody [EP734Y] images:

Customer reviews (feedback) regarding alpha Adducin antibody [EP734Y]

Customer FAQs regarding alpha Adducin antibody [EP734Y]

Protocols for alpha Adducin antibody [EP734Y]

Price and availability of products related to alpha Adducin antibody [EP734Y]

alpha Adducin antibody [EP734Y] - related products: