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Anti-alpha Adducin antibody [EP734Y] (ab40760)

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Overview

Product name

Anti-alpha Adducin antibody [EP734Y]
See all alpha Adducin products (10) ...

Description

Rabbit monoclonal [EP734Y] to alpha Adducin

Tested applications

WB, IHC-P, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide corresponding to residues in the C-term of human Adducin was used as immunogen.

Positive control

HeLa cell lysate, human lung (IHC-p).

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP734Y

Isotype

IgG

Research areas

Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin

  • Western blot - alpha Adducin antibody [EP734Y] (ab40760)Western blot - alpha Adducin antibody [EP734Y] (ab40760) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - alpha Adducin antibody [EP734Y] (ab40760)Immunohistochemistry (Paraffin-embedded sections) - alpha Adducin antibody [EP734Y] (ab40760) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - alpha Adducin antibody [EP734Y] (ab40760)Immunocytochemistry/ Immunofluorescence - alpha Adducin antibody [EP734Y] (ab40760) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab40760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: 1/250 - 1/500.
IHC-P: 1/250 - 1/500.

WB: 1/5000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).

Is unsuitable for FACS or IP.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

Tissue specificity

Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.

Sequence similarities

Belongs to the aldolase class II family. Adducin subfamily.

Domain

Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

Cellular localization

Cytoplasm > cytoskeleton. Cell membrane.

Target information above from: UniProt accessionP35611 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ADD 1 antibody
  • ADD A antibody
  • ADD1 antibody
  • ADDA antibody
  • ADDA_HUMAN antibody
  • Adducin 1 (alpha) antibody
  • Alpha adducin antibody
  • Alpha-adducin antibody
  • Erythrocyte adducin alpha subunit antibody
  • Erythrocyte adducin alpha subunit antibody
  • Erythrocyte adducin subunit alpha antibody
  • MGC3339 antibody
  • MGC44427 antibody
  • OTTHUMP00000151163 antibody
  • OTTHUMP00000151164 antibody
  • OTTHUMP00000151165 antibody
  • OTTHUMP00000218664 antibody
  • OTTHUMP00000218665 antibody
see all

Database links

see all

Anti-alpha Adducin antibody [EP734Y] images:

  Western blot - alpha Adducin antibody [EP734Y] (ab40760)

Western blot - alpha Adducin antibody [EP734Y] (ab40760)

Anti-alpha Adducin antibody [EP734Y] (ab40760) at 1/50000 dilution + HeLa cell lysate at 10 µg

Predicted band size : 120 kDa
Observed band size : 120 kDa

  Immunohistochemistry (Paraffin-embedded sections) - alpha Adducin antibody [EP734Y] (ab40760)

Immunohistochemistry (Paraffin-embedded sections) - alpha Adducin antibody [EP734Y] (ab40760)

ab40760 (1/250), staining human lung by immunohistochemistry, paraffin embedded tissue.

  Immunocytochemistry/ Immunofluorescence - alpha Adducin antibody [EP734Y] (ab40760)

Immunocytochemistry/ Immunofluorescence - alpha Adducin antibody [EP734Y] (ab40760)

ab40760, (1/250) staining HeLa cells by immunofluorescence.

References for Anti-alpha Adducin antibody [EP734Y] (ab40760)

ab40760 has not yet been referenced specifically in any publications.

Publishing research using ab40760? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"