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Western blot - alpha Adducin antibody (ab18314)
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Product Name
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alpha Adducin antibody
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See all alpha Adducin antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to alpha Adducin
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Immunogen
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Recombinant fragment spanning approximately 150aa in the N-terminus of the protein.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 81 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HepG2 cell extract.
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Cellular localization
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Cytoplasm, cytoskeleton and cell membrane
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Research areas
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Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
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Relevance
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Alpha adducin is a 80-120 kD member of the aldolase class II family and adducin subfamily. This protein has three isoforms that interact as heterodimers alpha/beta, and beta/gamma. The molecular weights for the various isoforms alpha=120 kD, beta=110kD; gamma=80kD. The alpha adducin protein is a ubiquitously expressed cytoskeletal protein that binds with high affinity to Ca2+/calmodulin and acts as a substrate for protein kinases A and C. This protein has been reported to promote the assembly of the spectrinactin network. Polymorphisms in alpha adducin are associated with cardiovascular disease and hypertension. Alpha adducin has been reported to interact with calmodulin. When phosphorylated by Rho-kinase, alpha adducin can regulate membrane ruffling and cell motility
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, Phosphate. pH 7.2 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this alpha Adducin antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for alpha Adducin antibody images, references, products related to ab18314 and other tools.
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alpha Adducin antibody images:
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 Western blot - alpha Adducin antibody (ab18314)
Predicted band size : 81 kDa
HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-a-adducin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
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References for alpha Adducin antibody (ab18314)
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This product has been used in: (two most recent references)
Tura A et al. Efficacy of Rho-kinase inhibition in promoting cell survival and reducing reactive gliosis in the rodent retina. Invest Ophthalmol Vis Sci 50:452-61 (2009). WB; Mouse. PubMed: 18757509
Fukata Y et al. Phosphorylation of adducin by Rho-kinase plays a crucial role in cell motility. J Cell Biol 145:347-61 (1999). PubMed: 10209029
If you publish research using ab18314 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to alpha Adducin
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alpha Adducin antibody - more information
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Customer reviews (feedback) regarding alpha Adducin antibody |
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