Anti-alpha Adducin antibody (ab51130)

Immunohistochemistry (Paraffin-embedded sections) - alpha Adducin antibody (ab51130)

This image shows paraffin-embedded human brain tissue stained with ab51130 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue

Western blot - alpha Adducin antibody (ab51130)

All lanes : Anti-alpha Adducin antibody (ab51130)

Lane 1 : Extracts from Hela cells treated with Forskolin (40nM, 30min) with no peptide
Lane 2 : Extracts from Hela cells treated with Forskolin (40nM, 30min) with with immunising peptide


Predicted band size : 81 kDa

Immunocytochemistry/ Immunofluorescence-alpha Adducin antibody(ab51130)

ICC/IF image of ab51130 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51130, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunoprecipitation - Anti-alpha Adducin antibody (ab51130)

ab51130 used in Immunoprecipitation and Western Blot.

Immunoprecipitation of NLN (EC 3.4.24.16), Thimet Oligopeptidase (thimet OP; EC 3.4.24.15), a-Adducin, and collapsin response mediator protein 2 (CRMP2) from photoradiolabeled P10 mouse forebrain membrane preparations. Immunoprecipitation of each protein was carried out from parallel nonspecific (NS) and total (Tot) photoradiolabeled groups.

a, equal amounts of NS and total sample of each protein immunoprecipitate were used to count the radioactive signal. Bck, background radioactivity; ***, p < 0.001 versus all other groups; n = 3–4).

b–e, representative Western blot confirmation of successful immunoprecipitation of each candidate protein from NS and total samples.

Image from Wangler NJ et al, J Biol Chem. 2012 Jan 2;287(1):114-22. Epub 2011 Oct 28, Fig 3. DOI 10.1074/jbc.M111.273052 January 2, 2012 The Journal of Biological Chemistry, 287, 114-122.