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Products:Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
Overview
Product name
Anti-alpha Adducin antibody
See all alpha Adducin products (10) ...
Description
Rabbit polyclonal to alpha Adducin
Specificity
ab51130 detects endogenous levels of total alpha Adducin protein.
Tested applications
IHC-P, WB, ELISA, ICC/IF, IPmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthesized non-phosphopeptide derived from human alpha Adducin around the phosphorylation site of serine 726 (T-P-SP-F-L).
Positive control
Human brain tissue or extracts from Hela cells treated with Forskolin.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000.Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
ELISA
ELISA: 1/40000ELISA: 1/40000
ICC/IF: Use a concentration of 1 - 5 µg/ml.
IP: Use at an assay dependent concentration. (PubMed: 22039052)
Target
Function
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Tissue specificity
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
Sequence similarities
Belongs to the aldolase class II family. Adducin subfamily.
Domain
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
Cellular localization
Cytoplasm > cytoskeleton. Cell membrane.
Target information above from: UniProt accessionP35611
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADD 1 antibody
- ADD A antibody
- ADD1 antibody
see all
Database links
- Entrez Gene: 118 Human
- Entrez Gene: 11518 Mouse
- Entrez Gene: 24170 Rat
- Omim: 102680 Human
- SwissProt: P35611 Human
- SwissProt: Q9QYC0 Mouse
- SwissProt: Q63028 Rat
- Unigene: 183706 Human
see all
Anti-alpha Adducin antibody images:
Immunohistochemistry (Paraffin-embedded sections) - alpha Adducin antibody (ab51130)
This image shows paraffin-embedded human brain tissue stained with ab51130 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue
Western blot - alpha Adducin antibody (ab51130)
All lanes : Anti-alpha Adducin antibody (ab51130)
Lane 1 : Extracts from Hela cells treated
with Forskolin (40nM, 30min) with no peptide
Lane 2 : Extracts from Hela cells treated
with Forskolin (40nM, 30min) with with immunising peptide
Predicted band size : 81 kDa
Immunocytochemistry/ Immunofluorescence-alpha Adducin antibody(ab51130)
ICC/IF image of ab51130 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51130, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunoprecipitation - Anti-alpha Adducin antibody (ab51130)
ab51130 used in Immunoprecipitation and Western Blot.Immunoprecipitation of NLN (EC 3.4.24.16), Thimet Oligopeptidase (thimet OP; EC 3.4.24.15), a-Adducin, and collapsin response mediator protein 2 (CRMP2) from photoradiolabeled P10 mouse forebrain membrane preparations. Immunoprecipitation of each protein was carried out from parallel nonspecific (NS) and total (Tot) photoradiolabeled groups.a, equal amounts of NS and total sample of each protein immunoprecipitate were used to count the radioactive signal. Bck, background radioactivity; ***, p < 0.001 versus all other groups; n = 3–4).b–e, representative Western blot confirmation of successful immunoprecipitation of each candidate protein from NS and total samples.
Image from Wangler NJ et al, J Biol Chem. 2012 Jan 2;287(1):114-22. Epub 2011 Oct 28, Fig 3. DOI 10.1074/jbc.M111.273052 January 2, 2012 The Journal of Biological Chemistry, 287, 114-122.
References for Anti-alpha Adducin antibody (ab51130)
This product has been referenced in:
- Wangler NJet al. Identification of Membrane-bound Variant of Metalloendopeptidase Neurolysin (EC 3.4.24.16) as the Non-angiotensin Type 1 (Non-AT1), Non-AT2 Angiotensin Binding Site. J Biol Chem 287:114-22 (2012). WB, IP; Mouse.Read more (PubMed: 22039052) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"