alpha Adducin (phospho S726) antibody (ab53093)

Product Name 

alpha Adducin (phospho S726) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to alpha Adducin (phospho S726)

Immunogen 

Synthetic phosphopeptide derived from human alpha Adducin around the phosphorylation site of serine 726 (TPS^PFL).

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab53093 detects endogenous levels of alpha Adducin only when phosphorylated at serine 726.

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/40000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: Human brain tissue
IHC-P: HeLa cell extract treated with Forskolin

Cellular localization 

Cytoplasm, cytoskeleton and cell membrane

Research areas 

Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin

Relevance 

Alpha adducin is a 80-120 kD member of the aldolase class II family and adducin subfamily. This protein has three isoforms that interact as heterodimers alpha/beta, and beta/gamma. The molecular weights for the various isoforms alpha=120 kD, beta=110kD; gamma=80kD. The alpha adducin protein is a ubiquitously expressed cytoskeletal protein that binds with high affinity to Ca²⁺/calmodulin and acts as a substrate for protein kinases A and C. This protein has been reported to promote the assembly of the spectrinactin network. Polymorphisms in alpha adducin are associated with cardiovascular disease and hypertension. Alpha adducin has been reported to interact with calmodulin. When phosphorylated by Rho-kinase, alpha adducin can regulate membrane ruffling and cell motility

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

ab53093 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

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References for alpha Adducin (phospho S726) antibody (ab53093)

  This product has been used in:

Tura A et al. Efficacy of Rho-kinase inhibition in promoting cell survival and reducing reactive gliosis in the rodent retina. Invest Ophthalmol Vis Sci 50:452-61 (2009). PubMed: 18757509

If you publish research using ab53093 please let us know so that we can cite the reference on this datasheet.

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