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Anti-alpha Adducin (phospho S726) antibody (ab53093)

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Overview

Product name

Anti-alpha Adducin (phospho S726) antibody
See all alpha Adducin products (10) ...

Description

Rabbit polyclonal to alpha Adducin (phospho S726)

Specificity

ab53093 detects endogenous levels of alpha Adducin only when phosphorylated at serine 726.

Tested applications

WB, IHC-P, ELISAmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic phosphopeptide derived from human alpha Adducin around the phosphorylation site of serine 726 (TPSPFL).

Positive control

WB: Human brain tissue IHC-P: HeLa cell extract treated with Forskolin

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab53093 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calmodulin Pathway
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Tags & Cell Markers >> Subcellular Markers >> Cytoskeleton >> Actin

  • Immunohistochemistry (Paraffin-embedded sections) - alpha Adducin (phospho S726) antibody (ab53093)Immunohistochemistry (Paraffin-embedded sections) - alpha Adducin (phospho S726) antibody (ab53093) image (enlarge)

  • Western blot - alpha Adducin (phospho S726) antibody (ab53093)Western blot - alpha Adducin (phospho S726) antibody (ab53093) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab53093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

  • ELISA

     ELISA: 1/40000

    ELISA: 1/40000

Target

Function

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

Tissue specificity

Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.

Sequence similarities

Belongs to the aldolase class II family. Adducin subfamily.

Domain

Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

Cellular localization

Cytoplasm > cytoskeleton. Cell membrane.

Target information above from: UniProt accessionP35611 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ADD 1 antibody
  • ADD A antibody
  • ADD1 antibody
  • ADD1 protein antibody
  • ADDA antibody
  • ADDA_HUMAN antibody
  • Adducin 1 (alpha) antibody
  • Adducin 1 alpha antibody
  • Alpha adducin antibody
  • Alpha-adducin antibody
  • Erythrocyte adducin alpha subunit antibody
  • Erythrocyte adducin subunit alpha antibody
  • MGC3339 antibody
  • MGC44427 antibody
  • OTTHUMP00000151163 antibody
  • OTTHUMP00000151164 antibody
  • OTTHUMP00000151165 antibody
  • OTTHUMP00000218664 antibody
  • OTTHUMP00000218665 antibody
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Database links

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Anti-alpha Adducin (phospho S726) antibody images:

  Immunohistochemistry (Paraffin-embedded sections) - alpha Adducin (phospho S726) antibody (ab53093)

Immunohistochemistry (Paraffin-embedded sections) - alpha Adducin (phospho S726) antibody (ab53093)

labelled with ab53093, at 1/50 dilution, staining alpha Adducin in paraffin embedded human brain tissue sections by Immunohistochemistry. Tissues had either been treated with (right image) or without (left image) the phosphopeptide.

  Western blot - alpha Adducin (phospho S726) antibody (ab53093)

Western blot - alpha Adducin (phospho S726) antibody (ab53093)

All lanes : Anti-alpha Adducin (phospho S726) antibody (ab53093) at 1/500 dilution

Lane 1 : HeLa cell extract treated with Forskolin (40nM, 30 mins) with phosphopeptide
Lane 2 : HeLa cell extract treated with Forskolin (40nM, 30 mins)


Predicted band size : 81 kDa
Observed band size : 81 kDa

References for Anti-alpha Adducin (phospho S726) antibody (ab53093)

This product has been referenced in:

  • Tura Aet al. Efficacy of Rho-kinase inhibition in promoting cell survival and reducing reactive gliosis in the rodent retina. Invest Ophthalmol Vis Sci 50:452-61 (2009).Read more (PubMed: 18757509) »

See 1 publication for this product

Publishing research using ab53093? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"