Anti-Alpha Amylase antibody (HRP) (ab34578)
Overview
- Product nameAnti-Alpha Amylase antibody (HRP)See all Alpha Amylase primary antibodies ...
- DescriptionRabbit polyclonal to Alpha Amylase (HRP)
- ConjugationHRP
- SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified alpha-Amylase [Bacillus amyloliquefaciens]. Cross reactivity against alpha-Amylase from other tissues and species is unknown.
- Tested applicationsWB, ELISA, Dot Blot, Electron Microscopy, IHC-P more details
- Species reactivityReacts with Bacillus amyloliquefaciens. not yet tested in other species.
- Immunogen
Full length protein: alpha Amylase (Bacillus amyloliquefaciens)
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2 -
Concentration information loading... - Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab34578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use at an assay dependent dilution. Predicted molecular weight: 57 kDa. |
| ELISA | ELISA: 1/2000 - 1/10000. |
| Dot Blot | Dot: Use at an assay dependent dilution. |
| Electron Microscopy | EM: Use at an assay dependent dilution. |
| IHC-P | IHC-P: Use at an assay dependent concentration. PubMed: 22584580 |
Target
- Sequence similaritiesBelongs to the glycosyl hydrolase 13 family.
- Cellular localizationSecreted.
Target information above from: UniProt accession
P04745
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 1 4 alpha D glucan glucanohydrolase antibody1 antibody4-alpha-D-glucan glucanohydrolase 1 antibody
- Alpha amylase 2B antibodyAlpha amylase carcinoid antibodyAlpha amylase carcinoid antibodyAlpha-amylase 1 antibodyAMY1_HUMAN antibodyAMY1C antibodyAMY2 antibodyAmylase alpha 2A; pancreatic antibodyPA antibodySalivary alpha-amylase antibody
see all
Anti-Alpha Amylase antibody (HRP) images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha Amylase antibody (HRP) (ab34578)Image from Asanuma-Date K et al., J Biol Chem. 2012 Jun 29;287(27):23104-18. Epub 2012 May 14.; Fig 1.; doi: 10.1074/jbc.M111.314658; June 29, 2012, The Journal of Biological Chemistry, 287, 23104-23118.Immunohistochemical analysis of formalin fixed, paraffin embedded porcine duodenum tissue from fasted animals. Duodenum sections were incubated in 10 µm PPA containing mannans at 4°C for 10 (left) and 30 minutes (right). Alpha Amylase is stained with ab34578 at 1/150 diluton.
References for Anti-Alpha Amylase antibody (HRP) (ab34578)
This product has been referenced in:
- Asanuma-Date K et al. Functional Regulation of Sugar Assimilation by N-Glycan-specific Interaction of Pancreatic a-Amylase with Glycoproteins of Duodenal Brush Border Membrane. J Biol Chem 287:23104-18 (2012). IHC-P ; Pig . Read more (PubMed: 22584580) »
