Overview

  • Product nameAnti-Alpha B Crystallin (phospho S19) antibodySee all Alpha B Crystallin primary antibodies ...
  • Description
    Rabbit polyclonal to Alpha B Crystallin (phospho S19)
  • Tested applicationsICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Sheep, Rabbit, Hamster, Pig, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide corresponding to Human Alpha B Crystallin aa 14-25.
    Sequence: FFPFHSpPSRLFD

  • Positive control
    • Immunofluorescence staining of phospho-alpha-B crystallin (Ser19) in U373 MG cells results in cytoplasmic staining.

Properties

Applications

Our Abpromise guarantee covers the use of ab5597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 20 kDa.

Target

  • FunctionMay contribute to the transparency and refractive index of the lens.
  • Tissue specificityLens as well as other tissues.
  • Involvement in diseaseDefects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.
  • Sequence similaritiesBelongs to the small heat shock protein (HSP20) family.
  • Cellular localizationCytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • AACRYA antibody
    • Alpha B crystallin antibody
    • Alpha crystallin B chain antibody
    • Alpha crystallin B chain antibody
    • Alpha(B) crystallin antibody
    • Alpha(B)-crystallin antibody
    • Alpha-crystallin B chain antibody
    • CRYA2 antibody
    • CRYAB antibody
    • CRYAB_HUMAN antibody
    • Crystallin alpha B antibody
    • Crystallin alpha polypeptide 2 antibody
    • CTPP 2 antibody
    • CTPP2 antibody
    • Heat shock 20 kD like protein antibody
    • Heat shock protein beta 5 antibody
    • Heat shock protein beta-5 antibody
    • HSPB5 antibody
    • NY REN 27 antigen antibody
    • Renal carcinoma antigen NY REN 27 antibody
    • Renal carcinoma antigen NY-REN-27 antibody
    • Rosenthal fiber component antibody
    see all

Anti-Alpha B Crystallin (phospho S19) antibody images

  • All lanes : Anti-Alpha B Crystallin (phospho S19) antibody (ab5597) at 0.5 µg/ml

    Lane 1 : phospho-alpha-B crystallin (Ser19)
    Lane 2 : unphosphorylated alpha-B crystallin


    Observed band size : 20 kDa (why is the actual band size different from the predicted?)

References for Anti-Alpha B Crystallin (phospho S19) antibody (ab5597)

This product has been referenced in:
  • Lambrecht S  et al. Differential expression of alphaB-crystallin and evidence of its role as a mediator of matrix gene expression in osteoarthritis. Arthritis Rheum 60:179-88 (2009). WB ; Human . Read more (PubMed: 19116904) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"