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- Secondary antibodies
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Product name
Anti-alpha B Crystallin (phospho S59) antibody
See all Alpha B Crystallin products (22) ...
Description
Rabbit polyclonal to alpha B Crystallin (phospho S59)
Specificity
This antibody does not detect the unphosphorylated form of the protein.
Tested applications
IHC-Fr, WB, IF, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Predicted to work with
Rabbit, Hamster
Immunogen
Synthetic peptide: FLRAPS(p)WIDTG, corresponding to amino acids 54-64 of Human Crystallin (alpha B).
FLRAPS(p)WIDTG
Positive control
U373 MG cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS, 30mg/ml BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Cancer >> Cancer Metabolism >> Cellular metabolic process
Neuroscience >> Neurotransmission >> Receptors / Channels >> Tyrosine Kinase Receptors
Neuroscience >> Sensory System >> Visual system
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent concentration. PubMed: 21126175
WB: Use a concentration of 0.5 µg/ml. Detects a band of approximately 20 kDa.
IF: Use a concentration of 8 µg/ml.
IHC-P: Use a concentration of 8 µg/ml.
Target
Function
May contribute to the transparency and refractive index of the lens.
Tissue specificity
Lens as well as other tissues.
Involvement in disease
Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.
Sequence similarities
Belongs to the small heat shock protein (HSP20) family.
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
Target information above from: UniProt accessionP02511
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alpha crystallin B chain antibody
- AACRYA antibody
- Alpha B crystallin antibody
see all
Database links
- Entrez Gene: 281719 Cow
- Entrez Gene: 1410 Human
- Entrez Gene: 12955 Mouse
- Entrez Gene: 100009295 Rabbit
- Entrez Gene: 25420 Rat
- Omim: 123590 Human
- SwissProt: P02510 Cow
- SwissProt: P02511 Human
see all
Anti-alpha B Crystallin (phospho S59) antibody images:
Western blot - Anti-alpha B Crystallin (phospho S59) antibody (ab5577)
ab5577 at 0.5µg/ml concentration staining approximately 20 KDa Crystallin Alpha B in lane 1) phospho-alpha-B crystallin (Ser59) and lane 2) non phosphorylated alpha-B crystallin in U373 cell lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - alpha B Crystallin (phospho S59) antibody (ab5577)
ab5577 staining of (top panel) human kidney tissue sections, highlighting highly localised staining in tubular epithelial cells, and (bottom panel) human ovarian carcinoma sections. Formalin-fixed paraffin embedded sections of human kidney and ovarian carcinoma tissue were incubated with ab5577 (1/1000) for 2 hours at room temperature. Antigen retrieval was performed by heat induction in citrate buffer, pH 6.
This image is courtesy of an Abreview submitted by Carl Hobbs, King's College London, UK
References for Anti-alpha B Crystallin (phospho S59) antibody (ab5577)
This product has been referenced in:
- Rajasekaran NS et al. Sustained activation of nuclear erythroid 2-related factor 2/antioxidant response element signaling promotes reductive stress in the human mutant protein aggregation cardiomyopathy in mice. Antioxid Redox Signal 14:957-71 (2011). WB, IHC-Fr; Mouse.Read more (PubMed: 21126175) »
See 1 publication for this product
Publishing research using ab5577? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"