Anti-alpha Defensin 5 antibody [8C8] (ab62757)
Key features and details
- Mouse monoclonal [8C8] to alpha Defensin 5
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-alpha Defensin 5 antibody [8C8]
See all alpha Defensin 5 primary antibodies -
Description
Mouse monoclonal [8C8] to alpha Defensin 5 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human duodenum FFPE tissue sections.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
8C8 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing. -
Tissue specificity
Paneth cells of the small intestine (at protein level). -
Sequence similarities
Belongs to the alpha-defensin family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. Cytoplasmic vesicle > secretory vesicle. Peptides HD5(20-94), HD5(23-94) and HD5(29-94) are found within tissues, HD5(20-94) being the predominant intracellular form. Peptides HD5(56-94) and HD5(63-94) are found in the extracellular milieu, HD5(63-94) being the most abundant form. - Information by UniProt
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Database links
- Entrez Gene: 1670 Human
- Omim: 600472 Human
- SwissProt: Q01523 Human
- Unigene: 655233 Human
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Alternative names
- alpha 5 antibody
- DEF5 antibody
- DEF5_HUMAN antibody
see all
Images
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IHC image of alpha Defensin 5 staining in human duodenum formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab62757, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab62757 has been referenced in 5 publications.
- Haberman Y et al. Age-of-diagnosis dependent ileal immune intensification and reduced alpha-defensin in older versus younger pediatric Crohn Disease patients despite already established dysbiosis. Mucosal Immunol 12:491-502 (2019). PubMed: 30542108
- Stronati L et al. Altered expression of innate immunity genes in different intestinal sites of children with ulcerative colitis. Dig Liver Dis 42:848-53 (2010). WB ; Human . PubMed: 20452301
- Arijs I et al. Mucosal gene expression of antimicrobial peptides in inflammatory bowel disease before and after first infliximab treatment. PLoS One 4:e7984 (2009). IHC-P ; Human . PubMed: 19956723
- Wehkamp J et al. Paneth cell antimicrobial peptides: topographical distribution and quantification in human gastrointestinal tissues. FEBS Lett 580:5344-50 (2006). PubMed: 16989824
- Wehkamp J et al. Reduced Paneth cell alpha-defensins in ileal Crohn's disease. Proc Natl Acad Sci U S A 102:18129-34 (2005). PubMed: 16330776