alpha Fetoprotein Human ELISA Kit (ab108631)


  • Product namealpha Fetoprotein Human ELISA Kit
    See all alpha Fetoprotein kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 2 ng/ml
  • Range
    2 ng/ml - 300 ng/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s alpha Fetoprotein (AFP) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of alpha Fetoprotein in Human serum.

    A 96-well plate has been precoated with anti-alpha Fetoprotein antibodies. Samples and standards and are added to the wells, where alpha Fetoprotein in the sample and standards binds to the precoated antibody. After incubation and washing, added Anti-alpha Fetoprotein-HRP conjugate binds to the antibody-alpha Fetoprotein complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of alpha Fetoprotein in the sample and the intensity is measured at 450 nm.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate


  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    alpha Fetoprotein Standard - 0 ng/mL 1 x 0.5ml
    alpha Fetoprotein Standard - 150 ng/mL 1 x 0.5ml
    alpha Fetoprotein Standard - 20 ng/mL 1 x 0.5ml
    alpha Fetoprotein Standard - 300 ng/mL 1 x 0.5ml
    alpha Fetoprotein Standard - 5 ng/mL 1 x 0.5ml
    alpha Fetoprotein Standard - 50 ng/mL 1 x 0.5ml
    Anti-alpha Fetoprotein Coated Microplate (12 x 8 wells) 1 unit
    Anti-alpha Fetoprotein HRP Conjugate 1 x 18ml
    Stop Solution 1 x 11ml
    TMB Substrate Solution 1 x 11ml
    Zero Buffer 1 x 13ml
  • Research Areas
  • FunctionBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • Tissue specificityPlasma. Synthesized by the fetal liver and yolk sac.
  • Sequence similaritiesBelongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Developmental stageOccurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • Post-translational
    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Afp
    • Alpha 1 fetoprotein
    • Alpha fetoglobulin
    • Alpha-1-fetoprotein
    • Alpha-fetoglobulin
    • Alpha-fetoprotein
    • FETA
    • HPAFP
    see all
  • Database links


Our Abpromise guarantee covers the use of ab108631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

alpha Fetoprotein Human ELISA Kit images

  • Representative standard curve using ab108631


References for alpha Fetoprotein Human ELISA Kit (ab108631)

ab108631 has not yet been referenced specifically in any publications.

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Thank you for contacting us. I can confirm that all the mentioned kits (ab99992, ab108631, ab108635, ab108838) come with a 96-well strip plate (12x8).  Each strip (12 total) has 8 wells so the customer can use a strip at a time if they wish. I ...

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