Overview

  • Product namealpha Fetoprotein Human ELISA Kit
    See all alpha Fetoprotein kits
  • Detection methodColorimetric
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    0.35 ng/ml
  • Range
    5 ng/ml - 200 ng/ml
  • Recovery

    98.33 %

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s alpha Fetoprotein Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of alpha Fetoprotein in serum and plasma.

     

    A 96-well plate has been precoated with anti-alpha Fetoprotein IgG antibodies. Samples, standards and the alpha Fetoprotein-HRP conjugate are added to the wells, where alpha Fetoprotein in the sample and standards binds to the precoated antibody and added alpha Fetoprotein-HRP conjugate binds to this antibody-AFP complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of alpha Fetoprotein in the sample and the intensity is measured at 450 nm.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    50X Washing Solution 1 x 20ml
    alpha Fetoprotein Standard 0 – 0 ng/mL 1 x 1ml
    alpha Fetoprotein Standard 1 – 5 ng/mL 1 x 1ml
    alpha Fetoprotein Standard 2 – 20 ng/mL 1 x 1ml
    alpha Fetoprotein Standard 3 – 80 ng/mL 1 x 1ml
    alpha Fetoprotein Standard 4– 200 ng/mL 1 x 1ml
    Anti-alpha Fetoprotein HRP Conjugate (Concentrated) 1 x 1ml
    Anti-alpha Fetoprotein IgG Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit
    Cover foil 1 unit
    Incubation buffer 1 x 50ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • Research Areas
  • Alternative names
    • AFP
    • Alpha 1 fetoprotein
    • Alpha fetoglobulin
    • FETA
    • HPAFP
    see all

Applications

Our Abpromise guarantee covers the use of ab108680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

alpha Fetoprotein Human ELISA Kit images

  • Representative Standard Curve using ab108680

Protocols

References for alpha Fetoprotein Human ELISA Kit (ab108680)

ab108680 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"