alpha Fetoprotein Human ELISA Kit (ab108680)
- Product namealpha Fetoprotein Human ELISA KitSee all alpha Fetoprotein kits ...
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Control Sera 48 <=6.4%
- Tests1 x 96 test
- Sample typeSerum, Plasma
- Assay typeSandwich (quantitative)
- Sensitivity0.35 ng/ml
- Range5 ng/ml - 200 ng/ml
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s alpha Fetoprotein Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of alpha Fetoprotein in serum and plasma.
A 96-well plate has been precoated with anti-alpha Fetoprotein IgG antibodies. Samples, standards and the alpha Fetoprotein-HRP conjugate are added to the wells, where alpha Fetoprotein in the sample and standards binds to the precoated antibody and added alpha Fetoprotein-HRP conjugate binds to this antibody-AFP complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of alpha Fetoprotein in the sample and the intensity is measured at 450 nm.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 50X Washing Solution 1 x 20ml alpha Fetoprotein Standard 0 – 0 ng/mL 1 x 1ml alpha Fetoprotein Standard 1 – 5 ng/mL 1 x 1ml alpha Fetoprotein Standard 2 – 20 ng/mL 1 x 1ml alpha Fetoprotein Standard 3 – 80 ng/mL 1 x 1ml alpha Fetoprotein Standard 4– 200 ng/mL 1 x 1ml Anti-alpha Fetoprotein HRP Conjugate (Concentrated) 1 x 1ml Anti-alpha Fetoprotein IgG Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Cover foil 1 unit Incubation buffer 1 x 50ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml
- Research Areas
- Alpha 1 fetoprotein
- Alpha fetoglobulin
Our Abpromise guarantee covers the use of ab108680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for alpha Fetoprotein Human ELISA Kit (ab108680)
ab108680 has not yet been referenced specifically in any publications.