alpha Fetoprotein Human ELISA Kit (ab108838)
- Product namealpha Fetoprotein Human ELISA KitSee all alpha Fetoprotein kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity= 0.15 ng/ml
- Range0.156 ng/ml - 10 ng/ml
- Assay time4h 0m
- Species reactivityReacts with: Human
- Product overview
Alpha Fetoprotein is a fetal-specific glycoprotein with a molecular weight of around 70 kDa. It is expressed in the embryonic liver, by cells of the vitelline sac and by the fetal intestinal tract in the first trimester of pregnancy. After birth the synthesis of alpha Fetoprotein decreases rapidly. Alpha Fetoprotein level in adults is low but detectable. Alpha Fetoprotein has no known function in healthy adults. A high level of alpha Fetoprotein in adult individuals may be associated with Hepatocellular carcinoma (HCC), malignant tumor of the liver. Thus, the concentration of alpha Fetoprotein in serum can be measured as a first step in HCC diagnosis.
ab108838 alpha Fetoprotein Human ELISA kit is designed for detection of human alpha Fetoprotein in plasma, serum and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures alpha Fetoprotein in less than 4 hours. A polyclonal antibody specific for alpha Fetoprotein has been pre-coated onto a microplate. Alpha Fetoprotein in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for alpha Fetoprotein, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the colour is measured.
This assay recognizes both natural and recombinant human alpha Fetoprotein.
- Tested applicationsELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Alpha-Fetoprotein Microplate 1 x 96 tests Alpha-Fetoprotein Standard 1 unit Biotinylated Alpha-Fetoprotein Antibody (50x) 1 x 140µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml
- Research Areas
- AFPAlpha 1 fetoproteinAlpha fetoglobulin
Our Abpromise guarantee covers the use of ab108838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for alpha Fetoprotein Human ELISA Kit (ab108838)
ab108838 has not yet been referenced specifically in any publications.