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Overview

  • Product nameAnti-Alpha Fodrin antibody [D8B7]
    See all Alpha Fodrin primary antibodies
  • Description
    Mouse monoclonal [D8B7] to Alpha Fodrin
  • Tested applicationsICC/IF, IP, IHC-P, ELISA, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human, African Green Monkey, Chinese Hamster
  • Immunogen

    Recombinant alpha Fodrin (non-erythroid spectrin) SH3 domain (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab11755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21059848
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. See Abreviews.
ELISA 1/100000 - 1/1e+006.
WB 1/100 - 1/1000. Predicted molecular weight: 297 kDa.
IHC-Fr 1/10 - 1/50.

Target

  • FunctionFodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
  • Involvement in diseaseDefects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
  • Sequence similaritiesBelongs to the spectrin family.
    Contains 3 EF-hand domains.
    Contains 1 SH3 domain.
    Contains 23 spectrin repeats.
  • Post-translational
    modifications
    Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • (ALPHA)II-SPECTRIN antibody
    • Alpha II Spectrin antibody
    • Alpha-II spectrin antibody
    • brain antibody
    • EIEE5 antibody
    • FLJ17738 antibody
    • FLJ44613 antibody
    • Fodrin alpha chain antibody
    • Fodrin, alpha antibody
    • NEAS antibody
    • Non erythrocytic spectrin alpha antibody
    • non-erythroid alpha chain antibody
    • SPECA antibody
    • Spectrin alpha chain antibody
    • Spectrin alpha chain brain antibody
    • Spectrin alpha non erythrocytic 1 antibody
    • Spectrin antibody
    • Spectrin non erythroid alpha chain antibody
    • Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody
    • Spectrin, nonerythroid, alpha subunit antibody
    • Spna2 antibody
    • SPTA 2 antibody
    • SPTA2 antibody
    • SPTA2_HUMAN antibody
    • SPTAN 1 antibody
    • SPTAN1 antibody
    see all

Anti-Alpha Fodrin antibody [D8B7] images

  • IF using ab11755.
  • ab11755 at 1/100 staining mouse gut (small bowel) tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was blocked and incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

  • Anti-Alpha Fodrin antibody [D8B7] (ab11755) at 1/1000 dilution + 3T3 whole cell lysate at 30 µg

    Secondary
    HRP conjugated goat anti-mouse at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 297 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Alpha Fodrin antibody [D8B7] (ab11755)

This product has been referenced in:
  • Duffy KR  et al. Investigation of cytoskeleton proteins in neurons of the cat lateral geniculate nucleus. J Comp Neurol 520:186-99 (2012). IHC-FoFr ; Cat . Read more (PubMed: 21800310) »
  • O'Connell DJ  et al. Integrated protein array screening and high throughput validation of 70 novel neural calmodulin-binding proteins. Mol Cell Proteomics 9:1118-32 (2010). WB ; Mouse . Read more (PubMed: 20068228) »

See all 5 Publications for this product

Product Wall

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing
Sample Sheep Tissue lysate - other (cardiac)
Specification cardiac
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Dec 12 2013

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Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa S3)
Specification HeLa S3
Immuno-precipitation step Protein G
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Submitted Oct 30 2007

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Application Immunoprecipitation
Sample Rat Cell lysate - whole cell (PC12)
Total protein in input 500 µg
Specification PC12
Immuno-precipitation step Protein G
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Submitted Oct 30 2007

Application Western blot
Sample Hamster Cell lysate - whole cell (CHO cell line)
Loading amount 30 µg
Specification CHO cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Sep 18 2007

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Application Western blot
Sample African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
Loading amount 30 µg
Specification Cos-7 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Sep 18 2007

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Application Western blot
Sample Mouse Cell lysate - whole cell (3T3 cell line)
Loading amount 30 µg
Specification 3T3 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Sep 18 2007

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (anal cancer)
Specification anal cancer
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 22°C
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Submitted Jul 31 2007

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (gut (small bowel))
Specification gut (small bowel)
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 22°C
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Submitted Jul 31 2007

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Application Western blot
Sample Rat Cell lysate - whole cell (PC12)
Loading amount 40 µg
Specification PC12
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jul 11 2007

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Application Western blot
Sample Human Cell lysate - whole cell (U2OS)
Loading amount 40 µg
Specification U2OS
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jul 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"