Overview

  • Product nameAnti-Alpha Fodrin antibody [D8B7]
    See all Alpha Fodrin primary antibodies
  • Description
    Mouse monoclonal [D8B7] to Alpha Fodrin
  • SpecificityClone D8B7 is developed against the alpha II spectrin SH3 domain (Xu et al., 2001). Clone D8B7 is a specific marker for human, rat and mouse alpha Fodrin or alpha II spectrin. In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin (reviewed in Bennett, 1993; Goodman, 1995 & De Matteis, 2000). It does not crossreact with alpha I (erythroid) spectrin SH3 domain.
  • Tested applicationsICC/IF, IP, IHC-P, ELISA, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human, African Green Monkey, Chinese Hamster
  • Immunogen

    Recombinant alpha Fodrin (non-erythroid spectrin) SH3 domain (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab11755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 21059848
IP Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution. See Abreviews.
ELISA 1/100000 - 1/1e+006.
WB 1/100 - 1/1000. Predicted molecular weight: 297 kDa.
IHC-Fr 1/10 - 1/50.

Target

  • FunctionFodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
  • Involvement in diseaseDefects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
  • Sequence similaritiesBelongs to the spectrin family.
    Contains 3 EF-hand domains.
    Contains 1 SH3 domain.
    Contains 23 spectrin repeats.
  • Post-translational
    modifications
    Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • (ALPHA)II-SPECTRIN antibody
    • Alpha II Spectrin antibody
    • Alpha-II spectrin antibody
    • brain antibody
    • EIEE5 antibody
    • FLJ17738 antibody
    • FLJ44613 antibody
    • Fodrin alpha chain antibody
    • Fodrin, alpha antibody
    • NEAS antibody
    • Non erythrocytic spectrin alpha antibody
    • non-erythroid alpha chain antibody
    • SPECA antibody
    • Spectrin alpha chain antibody
    • Spectrin alpha chain brain antibody
    • Spectrin alpha non erythrocytic 1 antibody
    • Spectrin antibody
    • Spectrin non erythroid alpha chain antibody
    • Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody
    • Spectrin, nonerythroid, alpha subunit antibody
    • Spna2 antibody
    • SPTA 2 antibody
    • SPTA2 antibody
    • SPTA2_HUMAN antibody
    • SPTAN 1 antibody
    • SPTAN1 antibody
    see all

Anti-Alpha Fodrin antibody [D8B7] images

  • IF using ab11755.
  • ab11755 at 1/100 staining mouse gut (small bowel) tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was blocked and incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

  • Anti-Alpha Fodrin antibody [D8B7] (ab11755) at 1/1000 dilution + 3T3 whole cell lysate at 30 µg

    Secondary
    HRP conjugated goat anti-mouse at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 297 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Alpha Fodrin antibody [D8B7] (ab11755)

This product has been referenced in:
  • Duffy KR  et al. Investigation of cytoskeleton proteins in neurons of the cat lateral geniculate nucleus. J Comp Neurol 520:186-99 (2012). IHC-FoFr ; Cat . Read more (PubMed: 21800310) »
  • O'Connell DJ  et al. Integrated protein array screening and high throughput validation of 70 novel neural calmodulin-binding proteins. Mol Cell Proteomics 9:1118-32 (2010). WB ; Mouse . Read more (PubMed: 20068228) »

See all 5 Publications for this product

Product Wall

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing
Sample Sheep Tissue lysate - other (cardiac)
Specification cardiac
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Dec 12 2013

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Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa S3)
Specification HeLa S3
Immuno-precipitation step Protein G
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Submitted Oct 30 2007

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Application Immunoprecipitation
Sample Rat Cell lysate - whole cell (PC12)
Total protein in input 500 µg
Specification PC12
Immuno-precipitation step Protein G
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Submitted Oct 30 2007

Application Western blot
Sample Hamster Cell lysate - whole cell (CHO cell line)
Loading amount 30 µg
Specification CHO cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Sep 18 2007

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Application Western blot
Sample African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
Loading amount 30 µg
Specification Cos-7 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Sep 18 2007

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Application Western blot
Sample Mouse Cell lysate - whole cell (3T3 cell line)
Loading amount 30 µg
Specification 3T3 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Sep 18 2007

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (anal cancer)
Specification anal cancer
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 22°C
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Submitted Jul 31 2007

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (gut (small bowel))
Specification gut (small bowel)
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 22°C
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Submitted Jul 31 2007

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Application Western blot
Sample Rat Cell lysate - whole cell (PC12)
Loading amount 40 µg
Specification PC12
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jul 11 2007

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Application Western blot
Sample Human Cell lysate - whole cell (U2OS)
Loading amount 40 µg
Specification U2OS
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jul 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"