Overview

  • Product name
    Anti-Alpha Fodrin antibody [D8B7]
    See all Alpha Fodrin primary antibodies
  • Description
    Mouse monoclonal [D8B7] to Alpha Fodrin
  • Tested applications
    Suitable for: ICC/IF, IP, IHC-P, ELISA, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human, African green monkey, Chinese hamster
  • Immunogen

    Recombinant alpha Fodrin (non-erythroid spectrin) SH3 domain (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab11755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21059848
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. See Abreviews.
ELISA 1/100000 - 1/1e+006.
WB 1/100 - 1/1000. Predicted molecular weight: 297 kDa.
IHC-Fr 1/10 - 1/50.

Target

  • Function
    Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
  • Involvement in disease
    Defects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
  • Sequence similarities
    Belongs to the spectrin family.
    Contains 3 EF-hand domains.
    Contains 1 SH3 domain.
    Contains 23 spectrin repeats.
  • Post-translational
    modifications
    Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • (ALPHA)II-SPECTRIN antibody
    • Alpha II Spectrin antibody
    • Alpha-II spectrin antibody
    • brain antibody
    • EIEE5 antibody
    • FLJ17738 antibody
    • FLJ44613 antibody
    • Fodrin alpha chain antibody
    • Fodrin, alpha antibody
    • NEAS antibody
    • Non erythrocytic spectrin alpha antibody
    • non-erythroid alpha chain antibody
    • SPECA antibody
    • Spectrin alpha chain antibody
    • Spectrin alpha chain brain antibody
    • Spectrin alpha non erythrocytic 1 antibody
    • Spectrin antibody
    • Spectrin non erythroid alpha chain antibody
    • Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody
    • Spectrin, nonerythroid, alpha subunit antibody
    • Spna2 antibody
    • SPTA 2 antibody
    • SPTA2 antibody
    • SPTA2_HUMAN antibody
    • SPTAN 1 antibody
    • SPTAN1 antibody
    see all

Anti-Alpha Fodrin antibody [D8B7] images

  • IF using ab11755.
  • ab11755 at 1/100 staining mouse gut (small bowel) tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was blocked and incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

  • Anti-Alpha Fodrin antibody [D8B7] (ab11755) at 1/1000 dilution + 3T3 whole cell lysate at 30 µg

    Secondary
    HRP conjugated goat anti-mouse at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 297 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Alpha Fodrin antibody [D8B7] (ab11755)

This product has been referenced in:
  • Yamamoto K  et al. The neuroprotective effect of latanoprost acts via klotho-mediated suppression of calpain activation after optic nerve transection. J Neurochem 140:495-508 (2017). WB . Read more (PubMed: 27859240) »
  • Liu M  et al. Heme-mediated apoptosis and fusion damage in BeWo trophoblast cells. Sci Rep 6:36193 (2016). Read more (PubMed: 27796349) »

See all 8 Publications for this product

Product Wall

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing
Sample
Sheep Tissue lysate - other (cardiac)
Specification
cardiac
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Dec 12 2013

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Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa S3)
Specification
HeLa S3
Immuno-precipitation step
Protein G
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Submitted Oct 30 2007

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Application
Immunoprecipitation
Sample
Rat Cell lysate - whole cell (PC12)
Total protein in input
500 µg
Specification
PC12
Immuno-precipitation step
Protein G
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Submitted Oct 30 2007

Application
Western blot
Sample
Hamster Cell lysate - whole cell (CHO cell line)
Loading amount
30 µg
Specification
CHO cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Sep 18 2007

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Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
Loading amount
30 µg
Specification
Cos-7 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Sep 18 2007

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Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3 cell line)
Loading amount
30 µg
Specification
3T3 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Sep 18 2007

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (anal cancer)
Specification
anal cancer
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 22°C
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Submitted Jul 31 2007

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (gut (small bowel))
Specification
gut (small bowel)
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 22°C
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Submitted Jul 31 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (PC12)
Loading amount
40 µg
Specification
PC12
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jul 11 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Loading amount
40 µg
Specification
U2OS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jul 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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