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alpha Glucosidase II protein (Tagged-His Tag) (ab91793)

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Overview

  • Product namealpha Glucosidase II protein (Tagged-His Tag)See all alpha Glucosidase II proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 103-944 of Human alpha Glucosidase II isoform 2 with N terminal His tag; 842 amino acids, 104kDa (95.5kDa predicted from sequence). UniProt ID = Q14697.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 68 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesIDELEPRRPRYRVPDVLVADPPIARLSVSGRDENSVELTM AEGPYKIILTARPFRL DLLEDRSLLLSVNARGLLEFEH QRAPRVSQGSKDPAEGDGAQPEETPRDGDKPEET QGKA EKDEPGAWEETFKTHSDSKPYGPMSVGLDFSLPGMEHVYG IPEHADNLRLKV TEGGEPYRLYNLDVFQYELYNPMALY GSVPVLLAHNPHRDLGIFWLNAAETWVDIS SNTAGKTL FGKMMDYLQGSGETPQTDVRWMSETGIIDVFLLLGPSISD VFRQYASL TGTQALPPLFSLGYHQSRWNYRDEADVLEV DQGFDDHNLPCDVIWLDIEHADGKRY FTWDPSRFPQPR TMLERLASKRRKLVAIVDPHIKVDSGYRVHEELRNLGLYV KTRD GSDYEGWCWPGSAGYPDFTNPTMRAWWANMFSYD NYEGSAPNLFVWNDMNEPSVFN GPEVTMLKDAQHYGGW EHRDVHNIYGLYVHMATADGLRQRSGGMERPFVLARAFFA GSQRFGAVWTGDNTAEWDHLKISIPMCLSLGLVGLSFC GADVGGFFKNPEPELLVR WYQMGAYQPFFRAHAHLDTG RREPWLLPSQHNDIIRDALGQRYSLLPFWYTLLYQA HR EGIPVMRPLWVQYPQDVTTFNIDDQYLLGDALLVHPVSDS GAHGVQVYLPGQGE VWYDIQSYQKHHGPQTLYLPVTLS SIPVFQRGGTIVPRWMRVRRSSECMKDDPITL FVALSP QGTAQGELFLDDGHTFNYQTRQEFLLRRFSFSGNTLVSSS ADPEGHFETP IWIERVVIIGAGKPAAVVLQTKGSPESR LSFQHDPETSVLVLRKPGINVASDWSIH LR
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91793 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Alpha glucosidase II alpha subunitAlpha-glucosidase 2G2AN
        GANABGANAB_HUMANGlu IIGlucosidase alpha neutral ABGlucosidase II alphaGlucosidase II subunit alphaGluIIKIAA0088Neutral alpha glucosidase ABNeutral alpha glucosidase AB precursorNeutral alpha-glucosidase AB
      see all
  • FunctionCleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins.
  • Tissue specificityDetected in placenta.
  • PathwayGlycan metabolism; N-glycan metabolism.
  • Sequence similaritiesBelongs to the glycosyl hydrolase 31 family.
  • Cellular localizationEndoplasmic reticulum. Golgi apparatus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

    • Target information above from: UniProt accession Q14697 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    alpha Glucosidase II protein (Tagged-His Tag) images

    • SDS-PAGE - alpha Glucosidase II protein (Tagged-His Tag) (ab91793)
      SDS-PAGE - alpha Glucosidase II protein (Tagged-His Tag) (ab91793)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for alpha Glucosidase II protein (Tagged-His Tag) (ab91793)

    ab91793 has not yet been referenced specifically in any publications.

    Publishing research using ab91793 ? Please let us know so that we can cite the reference in this datasheet

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